Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 21 February 2003:
Vol. 299. no. 5610, pp. 1225 - 1227
DOI: 10.1126/science.1081453
Prev | Table of Contents | Next

Reports

Impact of Genetic Manipulation on the Fitness of Anopheles stephensi Mosquitoes

Flaminia Catteruccia,1 H. Charles J. Godfray,12 Andrea Crisanti13*

Genetic modification of mosquitoes offers exciting possibilities for controlling malaria, but success will depend on how transformation affects the fitness of modified insects. The expression of an exogenous gene, the mutations caused by its insertion, and inbreeding while transformed lines are established can all lead to reductions in fitness. Factors influencing fitness were investigated in cage experiments with four lines of transgenic Anopheles stephensi, a vector species of human malaria. The results indicate direct costs of the introduced transgene in at least three out of the four lines, as well as an apparent cost of the inbreeding involved in making transgenic homozygotes.

1 Department of Biological Sciences, SAF Building, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
2 NERC Centre for Population Biology, Imperial College London, Silwood Park Campus, Ascot, Berkshire SL5 7PY, UK.
3 University of Perugia, Via del Giochetto, 06100 Perugia, Italy.
*   To whom correspondence should be addressed. Email: acrs{at}ic.ac.uk

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Fitness of Transgenic Anopheles stephensi Mosquitoes Expressing the SM1 Peptide under the Control of a Vitellogenin Promoter.
C. Li, M. T. Marrelli, G. Yan, and M. Jacobs-Lorena (2008)
J. Hered. 99, 275-282
   Abstract »    Full Text »    PDF »
Transgenic Mosquitoes and the Fight Against Malaria: Managing Technology Push in a Turbulent GMO World.
B. G. J. Knols, H. C. Bossin, W. R. Mukabana, and A. S. Robinson (2007)
Am J Trop Med Hyg 77, 232-242
   Abstract »    Full Text »    PDF »
A New Robust Diagnostic Polymerase Chain Reaction for Determining the Mating Status of Female Anopheles gambiae Mosquitoes.
K. R. Ng'habi, A. Horton, B. G. J. Knols, and G. C. Lanzaro (2007)
Am J Trop Med Hyg 77, 485-487
   Abstract »    Full Text »    PDF »
Nature beats nurture: a case study of the physiological fitness of free-living and laboratory-reared male Anopheles gambiae s.l..
B. J. Huho, K. R. Ng'habi, G. F. Killeen, G. Nkwengulila, B. G. J. Knols, and H. M. Ferguson (2007)
J. Exp. Biol. 210, 2939-2947
   Abstract »    Full Text »    PDF »
Bacteria of the genus Asaia stably associate with Anopheles stephensi, an Asian malarial mosquito vector.
G. Favia, I. Ricci, C. Damiani, N. Raddadi, E. Crotti, M. Marzorati, A. Rizzi, R. Urso, L. Brusetti, S. Borin, et al. (2007)
PNAS 104, 9047-9051
   Abstract »    Full Text »    PDF »
Transgenic malaria-resistant mosquitoes have a fitness advantage when feeding on Plasmodium-infected blood.
M. T. Marrelli, C. Li, J. L. Rasgon, and M. Jacobs-Lorena (2007)
PNAS 104, 5580-5583
   Abstract »    Full Text »    PDF »
Genetically Engineered Underdominance for Manipulation of Pest Populations: A Deterministic Model.
K. Magori and F. Gould (2006)
Genetics 172, 2613-2620
   Abstract »    Full Text »    PDF »
CAGE TRIALS USING AN ENDOGENOUS MEIOTIC DRIVE GENE IN THE MOSQUITO AEDES AEGYPTI TO PROMOTE POPULATION REPLACEMENT.
S.-J. CHA, A. MORI, D. D. CHADEE, and D. W. SEVERSON (2006)
Am J Trop Med Hyg 74, 62-68
   Abstract »    Full Text »    PDF »
Site-specific genomic targeting in Drosophila.
C. Horn and A. M. Handler (2005)
PNAS 102, 12483-12488
   Abstract »    Full Text »    PDF »
Multilevel Analyses of Genetic Differentiation in Anopheles gambiae s.s. Reveal Patterns of Gene Flow Important for Malaria-Fighting Mosquito Projects.
F. Tripet, G. Dolo, and G. C. Lanzaro (2005)
Genetics 169, 313-324
   Abstract »    Full Text »    PDF »
Innate immunity in the malaria vector Anopheles gambiae: comparative and functional genomics.
M. A. Osta, G. K. Christophides, D. Vlachou, and F. C. Kafatos (2004)
J. Exp. Biol. 207, 2551-2563
   Abstract »    Full Text »    PDF »
Fitness of Anopheline Mosquitoes Expressing Transgenes That Inhibit Plasmodium Development.
L. A. Moreira, J. Wang, F. H. Collins, and M. Jacobs-Lorena (2004)
Genetics 166, 1337-1341
   Abstract »    Full Text »    PDF »
Assessing fitness costs for transgenic Aedes aegypti expressing the GFP marker and transposase genes.
N. Irvin, M. S. Hoddle, D. A. O'Brochta, B. Carey, and P. W. Atkinson (2004)
PNAS 101, 891-896
   Abstract »    Full Text »    PDF »
Towards genetic manipulation of wild mosquito populations to combat malaria: advances and challenges.
M. A. Riehle, P. Srinivasan, C. K. Moreira, and M. Jacobs-Lorena (2003)
J. Exp. Biol. 206, 3809-3816
   Abstract »    Full Text »    PDF »
Stable and heritable gene silencing in the malaria vector Anopheles stephensi.
A. E. Brown, L. Bugeon, A. Crisanti, and F. Catteruccia (2003)
Nucleic Acids Res. 31, e85
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)