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Originally published in Science Express on 23 January 2003
Science 14 February 2003:
Vol. 299. no. 5609, pp. 1064 - 1067
DOI: 10.1126/science.1081328
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Reports

DNA Damage-Induced Replication Fork Regression and Processing in Escherichia coli

Justin Courcelle,* Janet R. Donaldson, Kin-Hoe Chow, Charmain T. Courcelle

DNA lesions that block replication are a primary cause of rearrangements, mutations, and lethality in all cells. After ultraviolet (UV)-induced DNA damage in Escherichia coli, replication recovery requires RecA and several other recF pathway proteins. To characterize the mechanism by which lesion-blocked replication forks recover, we used two-dimensional agarose gel electrophoresis to show that replication-blocking DNA lesions induce a transient reversal of the replication fork in vivo. The reversed replication fork intermediate is stabilized by RecA and RecF and is degraded by the RecQ-RecJ helicase-nuclease when these proteins are absent. We propose that fork regression allows repair enzymes to gain access to the replication-blocking lesion, allowing processive replication to resume once the blocking lesion is removed.

Department of Biological Sciences, Box GY, Mississippi State University, Mississippi State, MS 39762, USA.
*   To whom correspondence should be addressed. E-mail: jcourcelle{at}biology.msstate.edu

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