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Science 7 February 2003:
Vol. 299. no. 5608, pp. 906 - 909
DOI: 10.1126/science.1081147
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Reports

Passage of Heme-Iron Across the Envelope of Staphylococcus aureus

Sarkis K. Mazmanian,12* Eric P. Skaar,12* Andrew H. Gaspar,12 Munir Humayun,3 Piotr Gornicki,2 Joanna Jelenska,2 Andrzej Joachmiak,4 Dominique M. Missiakas,15 Olaf Schneewind12dagger

The cell wall envelope of Gram-positive pathogens functions as a scaffold for the attachment of virulence factors and as a sieve that prevents diffusion of molecules. Here the isd genes (iron-regulated surface determinant) of Staphylococcus aureus were found to encode factors responsible for hemoglobin binding and passage of heme-iron to the cytoplasm, where it acts as an essential nutrient. Heme-iron passage required two sortases that tether Isd proteins to unique locations within the cell wall. Thus, Isd appears to act as an import apparatus that uses cell wall-anchored proteins to relay heme-iron across the bacterial envelope.

1 Committee on Microbiology,
2 Department of Molecular Genetics and Cell Biology,
3 Department of Geophysical Sciences,
5 Department of Biochemistry and Molecular Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA.
4 Bioscience Division, Structural Biology Center, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: oschnee{at}delphi.bsd.uchicago.edu

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