Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 31 January 2003:
Vol. 299. no. 5607, pp. 719 - 721
DOI: 10.1126/science.1078694
Prev | Table of Contents | Next

Reports

Modulation of Heterochromatin Protein 1 Dynamics in Primary Mammalian Cells

Richard Festenstein,1* Stamatis N. Pagakis,2 Kyoko Hiragami,1 Debbie Lyon,3 Alain Verreault,3 Belaid Sekkali,4 Dimitris Kioussis4

Heterochromatin protein 1 (HP1beta ), a key component of condensed DNA, is strongly implicated in gene silencing and centromeric cohesion. Heterochromatin has been considered a static structure, stabilizing crucial aspects of nuclear organization and prohibiting access to transcription factors. We demonstrate here, by fluorescence recovery after photobleaching, that a green fluorescent protein-HP1beta fusion protein is highly mobile within both the euchromatin and heterochromatin of ex vivo resting murine T cells. Moreover, T cell activation greatly increased this mobility, indicating that such a process may facilitate (hetero)chromatin remodeling and permit access of epigenetic modifiers and transcription factors to the many genes that are consequently derepressed.

1 CSC Gene Control Mechanisms and Disease Group, Division of Medicine, Imperial College School of Medicine, Hammersmith Campus, Du Cane Road, London W12 ONN, UK.
2 Confocal and Image Analysis Laboratory, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
3 Chromosome Dynamics Laboratory, Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3LD, UK.
4 Division of Molecular Immunology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
*   To whom correspondence should be addressed. E-mail: r.festenstein{at}ic.ac.uk

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Human Immunodeficiency Virus Integration Efficiency and Site Selection in Quiescent CD4+ T Cells.
D. N. Vatakis, S. Kim, N. Kim, S. A. Chow, and J. A. Zack (2009)
J. Virol. 83, 6222-6233
   Abstract »    Full Text »    PDF »
Msc1 links dynamic Swi6/HP1 binding to cell fate determination.
R. J. Lawrence and T. A. Volpe (2009)
PNAS 106, 1163-1168
   Abstract »    Full Text »    PDF »
HP1-{beta} is required for development of the cerebral neocortex and neuromuscular junctions.
R. Aucott, J. Bullwinkel, Y. Yu, W. Shi, M. Billur, J. P. Brown, U. Menzel, D. Kioussis, G. Wang, I. Reisert, et al. (2008)
J. Cell Biol. 183, 597-606
   Abstract »    Full Text »    PDF »
Changes in the Distributions and Dynamics of Polycomb Repressive Complexes during Embryonic Stem Cell Differentiation.
X. Ren, C. Vincenz, and T. K. Kerppola (2008)
Mol. Cell. Biol. 28, 2884-2895
   Abstract »    Full Text »    PDF »
Analysis of Chromatin Structure of Genes Silenced by Heterochromatin in Trans.
P. Nisha, J. L. Plank, and A. K. Csink (2008)
Genetics 179, 359-373
   Abstract »    Full Text »    PDF »
Analysis of protein domains and Rett syndrome mutations indicate that multiple regions influence chromatin-binding dynamics of the chromatin-associated protein MECP2 in vivo.
A. Kumar, S. Kamboj, B. M. Malone, S. Kudo, J. L. Twiss, K. J. Czymmek, J. M. LaSalle, and N. C. Schanen (2008)
J. Cell Sci. 121, 1128-1137
   Abstract »    Full Text »    PDF »
Microplate-based chromatin immunoprecipitation method, Matrix ChIP: a platform to study signaling of complex genomic events.
S. Flanagin, J. D. Nelson, D. G. Castner, O. Denisenko, and K. Bomsztyk (2008)
Nucleic Acids Res. 36, e17
   Abstract »    Full Text »    PDF »
Plasticity of HP1 proteins in mammalian cells.
G. K. Dialynas, S. Terjung, J. P. Brown, R. L. Aucott, B. Baron-Luhr, P. B. Singh, and S. D. Georgatos (2007)
J. Cell Sci. 120, 3415-3424
   Abstract »    Full Text »    PDF »
Pericentromeric Heterochromatin Domains Are Maintained without Accumulation of HP1.
J. Mateos-Langerak, M. C. Brink, M. S. Luijsterburg, I. van der Kraan, R. van Driel, and P. J. Verschure (2007)
Mol. Biol. Cell 18, 1464-1471
   Abstract »    Full Text »    PDF »
Epigenetic regulation of facultative heterochromatinisation in Planococcus citri via the Me(3)K9H3-HP1-Me(3)K20H4 pathway.
S. Bongiorni, B. Pasqualini, M. Taranta, P. B. Singh, and G. Prantera (2007)
J. Cell Sci. 120, 1072-1080
   Abstract »    Full Text »    PDF »
Differences in protein mobility between pioneer versus follower growth cones.
R. P. Kulkarni, M. Bak-Maier, and S. E. Fraser (2007)
PNAS 104, 1207-1212
   Abstract »    Full Text »    PDF »
HP1 Binding to Chromatin Methylated at H3K9 Is Enhanced by Auxiliary Factors.
R. Eskeland, A. Eberharter, and A. Imhof (2007)
Mol. Cell. Biol. 27, 453-465
   Abstract »    Full Text »    PDF »
Human heterochromatin proteins form large domains containing KRAB-ZNF genes.
M. J. Vogel, L. Guelen, E. de Wit, D. P. Hupkes, M. Loden, W. Talhout, M. Feenstra, B. Abbas, A.-K. Classen, and B. van Steensel (2006)
Genome Res. 16, 1493-1504
   Abstract »    Full Text »    PDF »
Analysis of Human Histone H2AZ Deposition In Vivo Argues against Its Direct Role in Epigenetic Templating Mechanisms..
A. Viens, U. Mechold, F. Brouillard, C. Gilbert, P. Leclerc, and V. Ogryzko (2006)
Mol. Cell. Biol. 26, 5325-5335
   Abstract »    Full Text »    PDF »
Methylation-independent Binding to Histone H3 and Cell Cycle-dependent Incorporation of HP1beta into Heterochromatin.
G. K. Dialynas, D. Makatsori, N. Kourmouli, P. A. Theodoropoulos, K. McLean, S. Terjung, P. B. Singh, and S. D. Georgatos (2006)
J. Biol. Chem. 281, 14350-14360
   Abstract »    Full Text »    PDF »
Scanning Chromatin: a New Paradigm?.
K. van Holde and J. Zlatanova (2006)
J. Biol. Chem. 281, 12197-12200
   Full Text »    PDF »
Chromatin Decondensation and Nuclear Reprogramming by Nucleoplasmin.
H. Tamada, N. V. Thuan, P. Reed, D. Nelson, N. Katoku-Kikyo, J. Wudel, T. Wakayama, and N. Kikyo (2006)
Mol. Cell. Biol. 26, 1259-1271
   Abstract »    Full Text »    PDF »
Fast chromatin immunoprecipitation assay.
J. D. Nelson, O. Denisenko, P. Sova, and K. Bomsztyk (2006)
Nucleic Acids Res. 34, e2
   Abstract »    Full Text »    PDF »
RNAi-mediated Heterochromatin Assembly in Fission Yeast.
M. ZOFALL and S.I.S. GREWAL (2006)
Cold Spring Harb Symp Quant Biol 71, 487-496
   Abstract »    PDF »
Different Domains Control the Localization and Mobility of LIKE HETEROCHROMATIN PROTEIN1 in Arabidopsis Nuclei.
A. Zemach, Y. Li, H. Ben-Meir, M. Oliva, A. Mosquna, V. Kiss, Y. Avivi, N. Ohad, and G. Grafi (2006)
PLANT CELL 18, 133-145
   Abstract »    Full Text »    PDF »
Association of BMI1 with Polycomb Bodies Is Dynamic and Requires PRC2/EZH2 and the Maintenance DNA Methyltransferase DNMT1.
I. Hernandez-Munoz, P. Taghavi, C. Kuijl, J. Neefjes, and M. van Lohuizen (2005)
Mol. Cell. Biol. 25, 11047-11058
   Abstract »    Full Text »    PDF »
Partner-regulated interaction of IFN regulatory factor 8 with chromatin visualized in live macrophages.
L. Laricchia-Robbio, T. Tamura, T. Karpova, B. L. Sprague, J. G. McNally, and K. Ozato (2005)
PNAS 102, 14368-14373
   Abstract »    Full Text »    PDF »
Systems biology in the cell nucleus.
S. Gorski and T. Misteli (2005)
J. Cell Sci. 118, 4083-4092
   Abstract »    Full Text »    PDF »
Genome-wide HP1 binding in Drosophila: Developmental plasticity and genomic targeting signals.
E. de Wit, F. Greil, and B. van Steensel (2005)
Genome Res. 15, 1265-1273
   Abstract »    Full Text »    PDF »
Regulation of ubiquitin ligase dynamics by the nucleolus.
K. Mekhail, M. Khacho, A. Carrigan, R. R.J. Hache, L. Gunaratnam, and S. Lee (2005)
J. Cell Biol. 170, 733-744
   Abstract »    Full Text »    PDF »
A glue for heterochromatin maintenance: stable SUV39H1 binding to heterochromatin is reinforced by the SET domain.
I. M. Krouwels, K. Wiesmeijer, T. E. Abraham, C. Molenaar, N. P. Verwoerd, H. J. Tanke, and R. W. Dirks (2005)
J. Cell Biol. 170, 537-549
   Abstract »    Full Text »    PDF »
Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9.
M. Tachibana, J. Ueda, M. Fukuda, N. Takeda, T. Ohta, H. Iwanari, T. Sakihama, T. Kodama, T. Hamakubo, and Y. Shinkai (2005)
Genes & Dev. 19, 815-826
   Abstract »    Full Text »    PDF »
Mutations in the Nucleosome Core Enhance Transcriptional Silencing.
E. Y. Xu, X. Bi, M. J. Holland, D. E. Gottschling, and J. R. Broach (2005)
Mol. Cell. Biol. 25, 1846-1859
   Abstract »    Full Text »    PDF »
Dynamic GATA Factor Interplay at a Multicomponent Regulatory Region of the GATA-2 Locus.
M. L. Martowicz, J. A. Grass, M. E. Boyer, H. Guend, and E. H. Bresnick (2005)
J. Biol. Chem. 280, 1724-1732
   Abstract »    Full Text »    PDF »
Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities.
E. Lukasova, Z. Koristek, M. Falk, S. Kozubek, S. Grigoryev, M. Kozubek, V. Ondrej, and I. Kroupova (2005)
J. Leukoc. Biol. 77, 100-111
   Abstract »    Full Text »    PDF »
Epigenetics and cancer.
A. H. Lund and M. van Lohuizen (2004)
Genes & Dev. 18, 2315-2335
   Abstract »    Full Text »    PDF »
Global Nature of Dynamic Protein-Chromatin Interactions In Vivo: Three-Dimensional Genome Scanning and Dynamic Interaction Networks of Chromatin Proteins.
R. D. Phair, P. Scaffidi, C. Elbi, J. Vecerova, A. Dey, K. Ozato, D. T. Brown, G. Hager, M. Bustin, and T. Misteli (2004)
Mol. Cell. Biol. 24, 6393-6402
   Abstract »    Full Text »    PDF »
Sir Antagonist 1 (San1) Is a Ubiquitin Ligase.
A. Dasgupta, K. L. Ramsey, J. S. Smith, and D. T. Auble (2004)
J. Biol. Chem. 279, 26830-26838
   Abstract »    Full Text »    PDF »
Resting CD4+ T Cells from Human Immunodeficiency Virus Type 1 (HIV-1)-Infected Individuals Carry Integrated HIV-1 Genomes within Actively Transcribed Host Genes.
Y. Han, K. Lassen, D. Monie, A. R. Sedaghat, S. Shimoji, X. Liu, T. C. Pierson, J. B. Margolick, R. F. Siliciano, and J. D. Siliciano (2004)
J. Virol. 78, 6122-6133
   Abstract »    Full Text »    PDF »
High- and Low-mobility Populations of HP1 in Heterochromatin of Mammalian Cells.
L. Schmiedeberg, K. Weisshart, S. Diekmann, G. Meyer zu Hoerste, and P. Hemmerich (2004)
Mol. Biol. Cell 15, 2819-2833
   Abstract »    Full Text »    PDF »
The Schizosaccharomyces pombe HIRA-Like Protein Hip1 Is Required for the Periodic Expression of Histone Genes and Contributes to the Function of Complex Centromeres.
C. Blackwell, K. A. Martin, A. Greenall, A. Pidoux, R. C. Allshire, and S. K. Whitehall (2004)
Mol. Cell. Biol. 24, 4309-4320
   Abstract »    Full Text »    PDF »
Regulation of Immature Protein Dynamics in the Endoplasmic Reticulum.
A. Kamada, H. Nagaya, T. Tamura, M. Kinjo, H.-Y. Jin, T. Yamashita, K. Jimbow, H. Kanoh, and I. Wada (2004)
J. Biol. Chem. 279, 21533-21542
   Abstract »    Full Text »    PDF »
Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells.
C. Molenaar, A. Abdulle, A. Gena, H. J. Tanke, and R. W. Dirks (2004)
J. Cell Biol. 165, 191-202
   Abstract »    Full Text »    PDF »
In Vivo Dynamics of Swi6 in Yeast: Evidence for a Stochastic Model of Heterochromatin.
T. Cheutin, S. A. Gorski, K. M. May, P. B. Singh, and T. Misteli (2004)
Mol. Cell. Biol. 24, 3157-3167
   Abstract »    Full Text »    PDF »
Components of a Pathway Maintaining Histone Modification and Heterochromatin Protein 1 Binding at the Pericentric Heterochromatin in Mammalian Cells.
H. Xin, H.-G. Yoon, P. B. Singh, J. Wong, and J. Qin (2004)
J. Biol. Chem. 279, 9539-9546
   Abstract »    Full Text »    PDF »
Localisation of histone macroH2A1.2 to the XY-body is not a response to the presence of asynapsed chromosome axes.
S. Hoyer-Fender, E. Czirr, R. Radde, J. M. A. Turner, S. K. Mahadevaiah, J. R. Pehrson, and P. S. Burgoyne (2004)
J. Cell Sci. 117, 189-198
   Abstract »    Full Text »    PDF »
Epigenetics, Histone H3 Variants, and the Inheritance of Chromatin States.
S. HENIKOFF, E. MCKITTRICK, and K. AHMAD (2004)
Cold Spring Harb Symp Quant Biol 69, 235-244
   Abstract »    PDF »
RNA Interference and Epigenetic Control of Heterochromatin Assembly in Fission Yeast.
H. CAM and S.I.S. GREWAL (2004)
Cold Spring Harb Symp Quant Biol 69, 419-428
   Abstract »    PDF »
Heterochromatingets dynamic.
W. A. Wells (2003)
J. Cell Biol. 160, 626
   Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)