Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 17 January 2003:
Vol. 299. no. 5605, pp. 396 - 399
DOI: 10.1126/science.1078540
Prev | Table of Contents | Next

Reports

Role of Anthocyanidin Reductase, Encoded by BANYULS in Plant Flavonoid Biosynthesis

De-Yu Xie,1 Shashi B. Sharma,1 Nancy L. Paiva,1 Daneel Ferreira,2 Richard A. Dixon1*

Condensed tannins (CTs) are flavonoid oligomers, many of which have beneficial effects on animal and human health. The flavanol (-)-epicatechin is a component of many CTs and contributes to flavor and astringency in tea and wine. We show that the BANYULS (BAN) genes from Arabidopsis thaliana and Medicago truncatula encode anthocyanidin reductase, which converts anthocyanidins to their corresponding 2,3-cis-flavan-3-ols. Ectopic expression of BAN in tobacco flower petals and Arabidopsis leaves results in loss of anthocyanins and accumulation of CTs.

1 Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
2 National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
*   To whom correspondence should be addressed. E-mail: radixon{at}noble.org

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A WD40 Repeat Protein from Medicago truncatula Is Necessary for Tissue-Specific Anthocyanin and Proanthocyanidin Biosynthesis But Not for Trichome Development.
Y. Pang, J. P. Wenger, K. Saathoff, G. J. Peel, J. Wen, D. Huhman, S. N. Allen, Y. Tang, X. Cheng, M. Tadege, et al. (2009)
Plant Physiology 151, 1114-1129
   Abstract »    Full Text »    PDF »
MATE Transporters Facilitate Vacuolar Uptake of Epicatechin 3'-O-Glucoside for Proanthocyanidin Biosynthesis in Medicago truncatula and Arabidopsis.
J. Zhao and R. A. Dixon (2009)
PLANT CELL 21, 2323-2340
   Abstract »    Full Text »    PDF »
Colloquium Papers: Adaptive radiations: From field to genomic studies.
S. A. Hodges and N. J. Derieg (2009)
PNAS 106, 9947-9954
   Abstract »    Full Text »    PDF »
Differential display mediated cloning of anthocyanidin reductase gene from tea (Camellia sinensis) and its relationship with the concentration of epicatechins.
K. Singh, A. Rani, A. Paul, S. Dutt, R. Joshi, A. Gulati, P. S. Ahuja, and S. Kumar (2009)
Tree Physiol 29, 837-846
   Abstract »    Full Text »    PDF »
Advancing Genetic Theory and Application by Metabolic Quantitative Trait Loci Analysis.
DanielJ. Kliebenstein (2009)
PLANT CELL 21, 1637-1646
   Abstract »    Full Text »    PDF »
The Wound-, Pathogen-, and Ultraviolet B-Responsive MYB134 Gene Encodes an R2R3 MYB Transcription Factor That Regulates Proanthocyanidin Synthesis in Poplar.
R. D. Mellway, L. T. Tran, M. B. Prouse, M. M. Campbell, and C. P. Constabel (2009)
Plant Physiology 150, 924-941
   Abstract »    Full Text »    PDF »
Occurrence of Condensed Tannins in Wheat and Feasibility for Reducing Pasture Bloat.
C. T. MacKown, B. F. Carver, and J. T. Edwards (2008)
Crop Sci. 48, 2470-2480
   Abstract »    Full Text »    PDF »
The Transcription Factor VvMYB5b Contributes to the Regulation of Anthocyanin and Proanthocyanidin Biosynthesis in Developing Grape Berries.
L. Deluc, J. Bogs, A. R. Walker, T. Ferrier, A. Decendit, J.-M. Merillon, S. P. Robinson, and F. Barrieu (2008)
Plant Physiology 147, 2041-2053
   Abstract »    Full Text »    PDF »
Profile of Richard Dixon.
M. Marino (2008)
PNAS 105, 2263-2265
   Full Text »    PDF »
Early Steps in Proanthocyanidin Biosynthesis in the Model Legume Medicago truncatula.
Y. Pang, G. J. Peel, E. Wright, Z. Wang, and R. A. Dixon (2007)
Plant Physiology 145, 601-615
   Abstract »    Full Text »    PDF »
The Arabidopsis MATE Transporter TT12 Acts as a Vacuolar Flavonoid/H+-Antiporter Active in Proanthocyanidin-Accumulating Cells of the Seed Coat.
K. Marinova, L. Pourcel, B. Weder, M. Schwarz, D. Barron, J.-M. Routaboul, I. Debeaujon, and M. Klein (2007)
PLANT CELL 19, 2023-2038
   Abstract »    Full Text »    PDF »
The Grapevine Transcription Factor VvMYBPA1 Regulates Proanthocyanidin Synthesis during Fruit Development.
J. Bogs, F. W. Jaffe, A. M. Takos, A. R. Walker, and S. P. Robinson (2007)
Plant Physiology 143, 1347-1361
   Abstract »    Full Text »    PDF »
Ectopic Expression of a Basic Helix-Loop-Helix Gene Transactivates Parallel Pathways of Proanthocyanidin Biosynthesis. Structure, Expression Analysis, and Genetic Control of Leucoanthocyanidin 4-Reductase and Anthocyanidin Reductase Genes in Lotus corniculatus.
F. Paolocci, M. P. Robbins, L. Madeo, S. Arcioni, S. Martens, and F. Damiani (2007)
Plant Physiology 143, 504-516
   Abstract »    Full Text »    PDF »
Expression of VvmybA1 Gene and Anthocyanin Accumulation in Various Grape Organs.
S.-T. Jeong, N. Goto-Yamamoto, K. Hashizume, S. Kobayashi, and M. Esaka (2006)
Am. J. Enol. Vitic. 57, 507-510
   Abstract »    Full Text »    PDF »
Phenolics and Ripening in Grape Berries.
D. O. Adams (2006)
Am. J. Enol. Vitic. 57, 249-256
   Abstract »    Full Text »    PDF »
Cultural Practice and Environmental Impacts on the Flavonoid Composition of Grapes and Wine: A Review of Recent Research.
M. O. Downey, N. K. Dokoozlian, and M. P. Krstic (2006)
Am. J. Enol. Vitic. 57, 257-268
   Abstract »    Full Text »    PDF »
Mechanistic Study on the Oxidation of Anthocyanidin Synthase by Quantum Mechanical Calculation.
J.-i. Nakajima, Y. Sato, T. Hoshino, M. Yamazaki, and K. Saito (2006)
J. Biol. Chem. 281, 21387-21398
   Abstract »    Full Text »    PDF »
Identification of Genes with Potential Roles in Apple Fruit Development and Biochemistry through Large-Scale Statistical Analysis of Expressed Sequence Tags.
S. Park, N. Sugimoto, M. D. Larson, R. Beaudry, and S. van Nocker (2006)
Plant Physiology 141, 811-824
   Abstract »    Full Text »    PDF »
Characterization of a Grapevine R2R3-MYB Transcription Factor That Regulates the Phenylpropanoid Pathway.
L. Deluc, F. Barrieu, C. Marchive, V. Lauvergeat, A. Decendit, T. Richard, J.-P. Carde, J.-M. Merillon, and S. Hamdi (2006)
Plant Physiology 140, 499-511
   Abstract »    Full Text »    PDF »
Anthocyanidin Reductase Gene Expression and Accumulation of Flavan-3-ols in Grape Berry.
A. Fujita, N. Soma, N. Goto-Yamamoto, H. Shindo, T. Kakuta, T. Koizumi, and K. Hashizume (2005)
Am. J. Enol. Vitic. 56, 336-342
   Abstract »    Full Text »    PDF »
Proanthocyanidin Synthesis and Expression of Genes Encoding Leucoanthocyanidin Reductase and Anthocyanidin Reductase in Developing Grape Berries and Grapevine Leaves.
J. Bogs, M. O. Downey, J. S. Harvey, A. R. Ashton, G. J. Tanner, and S. P. Robinson (2005)
Plant Physiology 139, 652-663
   Abstract »    Full Text »    PDF »
Genome-Wide Analysis of Hydrogen Peroxide-Regulated Gene Expression in Arabidopsis Reveals a High Light-Induced Transcriptional Cluster Involved in Anthocyanin Biosynthesis.
S. Vanderauwera, P. Zimmermann, S. Rombauts, S. Vandenabeele, C. Langebartels, W. Gruissem, D. Inze, and F. Van Breusegem (2005)
Plant Physiology 139, 806-821
   Abstract »    Full Text »    PDF »
Light and an exogenous transcription factor qualitatively and quantitatively affect the biosynthetic pathway of condensed tannins in Lotus corniculatus leaves.
F. Paolocci, T. Bovone, N. Tosti, S. Arcioni, and F. Damiani (2005)
J. Exp. Bot. 56, 1093-1103
   Abstract »    Full Text »    PDF »
A plasma membrane H+-ATPase is required for the formation of proanthocyanidins in the seed coat endothelium of Arabidopsis thaliana.
I. R. Baxter, J. C. Young, G. Armstrong, N. Foster, N. Bogenschutz, T. Cordova, W. A. Peer, S. P. Hazen, A. S. Murphy, and J. F. Harper (2005)
PNAS 102, 2649-2654
   Abstract »    Full Text »    PDF »
Expression of antiapoptotic genes bcl-xL and ced-9 in tomato enhances tolerance to viral-induced necrosis and abiotic stress.
P. Xu, S. J. Rogers, and M. J. Roossinck (2004)
PNAS 101, 15805-15810
   Abstract »    Full Text »    PDF »
Transcriptional Programs of Early Reproductive Stages in Arabidopsis.
L. Hennig, W. Gruissem, U. Grossniklaus, and C. Kohler (2004)
Plant Physiology 135, 1765-1775
   Abstract »    Full Text »    PDF »
Molecular and Biochemical Analysis of Two cDNA Clones Encoding Dihydroflavonol-4-Reductase from Medicago truncatula.
D.-Y. Xie, L. A. Jackson, J. D. Cooper, D. Ferreira, and N. L. Paiva (2004)
Plant Physiology 134, 979-994
   Abstract »    Full Text »    PDF »
Proanthocyanidin-Accumulating Cells in Arabidopsis Testa: Regulation of Differentiation and Role in Seed Development.
I. Debeaujon, N. Nesi, P. Perez, M. Devic, O. Grandjean, M. Caboche, and L. Lepiniec (2003)
PLANT CELL 15, 2514-2531
   Abstract »    Full Text »    PDF »
Communication between the Maternal Testa and the Embryo and/or Endosperm Affect Testa Attributes in Tomato.
A. B. Downie, D. Zhang, L. M.A. Dirk, R. R. Thacker, J. A. Pfeiffer, J. L. Drake, A. A. Levy, D. A. Butterfield, J. W. Buxton, and J. C. Snyder (2003)
Plant Physiology 133, 145-160
   Abstract »    Full Text »    PDF »
Proanthocyanidin Biosynthesis in Plants: PURIFICATION OF LEGUME LEUCOANTHOCYANIDIN REDUCTASE AND MOLECULAR CLONING OF ITS cDNA.
G. J. Tanner, K. T. Francki, S. Abrahams, J. M. Watson, P. J. Larkin, and A. R. Ashton (2003)
J. Biol. Chem. 278, 31647-31656
   Abstract »    Full Text »    PDF »
Expression of Anthocyanins and Proanthocyanidins after Transformation of Alfalfa with Maize Lc.
H. Ray, M. Yu, P. Auser, L. Blahut-Beatty, B. McKersie, S. Bowley, N. Westcott, B. Coulman, A. Lloyd, and M. Y. Gruber (2003)
Plant Physiology 132, 1448-1463
   Abstract »    Full Text »    PDF »
Legume Natural Products: Understanding and Manipulating Complex Pathways for Human and Animal Health.
R. A. Dixon and L. W. Sumner (2003)
Plant Physiology 131, 878-885
   Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)