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Science 10 January 2003:
Vol. 299. no. 5604, pp. 262 - 265
DOI: 10.1126/science.1078973
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Reports

Role of a Highly Conserved Bacterial Protein in Outer Membrane Protein Assembly

Romé Voulhoux, Martine P. Bos, Jeroen Geurtsen, Maarten Mols, Jan Tommassen*

After transport across the cytoplasmic membrane, bacterial outer membrane proteins are assembled into the outer membrane. Meningococcal Omp85 is a highly conserved protein in Gram-negative bacteria, and its homolog Toc75 is a component of the chloroplast protein-import machinery. Omp85 appeared to be essential for viability, and unassembled forms of various outer membrane proteins accumulated upon Omp85 depletion. Immunofluorescence microscopy revealed decreased surface exposure of outer membrane proteins, which was particularly apparent at the cell-division planes. Thus, Omp85 is likely to play a role in outer membrane protein assembly.

Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, Netherlands.
*   To whom correspondence should be addressed. E-mail: j.p.m.tommassen{at}bio.uu.nl

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S.-L. Tu, L.-J. Chen, M. D. Smith, Y.-s. Su, D. J. Schnell, and H.-m. Li (2004)
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J. J. Velarde and J. P. Nataro (2004)
J. Biol. Chem. 279, 31495-31504
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Three Homologues, Including Two Membrane-bound Proteins, of the Disulfide Oxidoreductase DsbA in Neisseria meningitidis: EFFECTS ON BACTERIAL GROWTH AND BIOGENESIS OF FUNCTIONAL TYPE IV PILI.
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J. Biol. Chem. 279, 27078-27087
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Identification of an outer membrane protein required for the transport of lipopolysaccharide to the bacterial cell surface.
M. P. Bos, B. Tefsen, J. Geurtsen, and J. Tommassen (2004)
PNAS 101, 9417-9422
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Isolation of Outer Membrane of Synechocystis sp. PCC 6803 and Its Proteomic Characterization.
F. Huang, E. Hedman, C. Funk, T. Kieselbach, W. P. Schroder, and B. Norling (2004)
Mol. Cell. Proteomics 3, 586-595
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N. Wiedemann, K. N. Truscott, S. Pfannschmidt, B. Guiard, C. Meisinger, and N. Pfanner (2004)
J. Biol. Chem. 279, 18188-18194
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Ancient Invasions: From Endosymbionts to Organelles.
S. D. Dyall, M. T. Brown, and P. J. Johnson (2004)
Science 304, 253-257
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The Protein Import Machinery of Mitochondria.
N. Wiedemann, A. E. Frazier, and N. Pfanner (2004)
J. Biol. Chem. 279, 14473-14476
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The Omp85 family of proteins is essential for outer membrane biogenesis in mitochondria and bacteria.
I. Gentle, K. Gabriel, P. Beech, R. Waller, and T. Lithgow (2004)
J. Cell Biol. 164, 19-24
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Sequence-specific Dimerization of the Transmembrane Domain of the "BH3-only" Protein BNIP3 in Membranes and Detergent.
E. S. Sulistijo, T. M. Jaszewski, and K. R. MacKenzie (2003)
J. Biol. Chem. 278, 51950-51956
   Abstract »    Full Text »    PDF »
An Essential Role of Sam50 in the Protein Sorting and Assembly Machinery of the Mitochondrial Outer Membrane.
V. Kozjak, N. Wiedemann, D. Milenkovic, C. Lohaus, H. E. Meyer, B. Guiard, C. Meisinger, and N. Pfanner (2003)
J. Biol. Chem. 278, 48520-48523
   Abstract »    Full Text »    PDF »
Molecular Basis of Bacterial Outer Membrane Permeability Revisited.
H. Nikaido (2003)
Microbiol. Mol. Biol. Rev. 67, 593-656
   Abstract »    Full Text »    PDF »


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