Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 10 January 2003:
Vol. 299. no. 5604, pp. 262 - 265
DOI: 10.1126/science.1078973
Prev | Table of Contents | Next

Reports

Role of a Highly Conserved Bacterial Protein in Outer Membrane Protein Assembly

Romé Voulhoux, Martine P. Bos, Jeroen Geurtsen, Maarten Mols, Jan Tommassen*

After transport across the cytoplasmic membrane, bacterial outer membrane proteins are assembled into the outer membrane. Meningococcal Omp85 is a highly conserved protein in Gram-negative bacteria, and its homolog Toc75 is a component of the chloroplast protein-import machinery. Omp85 appeared to be essential for viability, and unassembled forms of various outer membrane proteins accumulated upon Omp85 depletion. Immunofluorescence microscopy revealed decreased surface exposure of outer membrane proteins, which was particularly apparent at the cell-division planes. Thus, Omp85 is likely to play a role in outer membrane protein assembly.

Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, Netherlands.
*   To whom correspondence should be addressed. E-mail: j.p.m.tommassen{at}bio.uu.nl

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification, Characterization, and Molecular Application of a Virulence-Associated Autotransporter from a Pathogenic Pseudomonas fluorescens Strain.
Y.-h. Hu, C.-s. Liu, J.-h. Hou, and L. Sun (2009)
Appl. Envir. Microbiol. 75, 4333-4340
   Abstract »    Full Text »    PDF »
Folding and trimerization of signal sequence-less mature TolC in the cytoplasm of Escherichia coli.
M. Masi, G. Duret, A. H. Delcour, and R. Misra (2009)
Microbiology 155, 1847-1857
   Abstract »    Full Text »    PDF »
Investigation of the role of the BAM complex and SurA chaperone in outer-membrane protein biogenesis and type III secretion system expression in Salmonella.
Y. Fardini, J. Trotereau, E. Bottreau, C. Souchard, P. Velge, and I. Virlogeux-Payant (2009)
Microbiology 155, 1613-1622
   Abstract »    Full Text »    PDF »
Identification of neisserial DNA binding components.
E. Lang, K. Haugen, B. Fleckenstein, H. Homberset, S. A. Frye, O. H. Ambur, and T. Tonjum (2009)
Microbiology 155, 852-862
   Abstract »    Full Text »    PDF »
Signals in bacterial {beta}-barrel proteins are functional in eukaryotic cells for targeting to and assembly in mitochondria.
D. M. Walther, D. Papic, M. P. Bos, J. Tommassen, and D. Rapaport (2009)
PNAS 106, 2531-2536
   Abstract »    Full Text »    PDF »
Verification of a topology model of PorT as an integral outer-membrane protein in Porphyromonas gingivalis.
K.-A. Nguyen, J. Zylicz, P. Szczesny, A. Sroka, N. Hunter, and J. Potempa (2009)
Microbiology 155, 328-337
   Abstract »    Full Text »    PDF »
The Omp85-Related Chloroplast Outer Envelope Protein OEP80 Is Essential for Viability in Arabidopsis.
R. Patel, S.-C. Hsu, J. Bedard, K. Inoue, and P. Jarvis (2008)
Plant Physiology 148, 235-245
   Abstract »    Full Text »    PDF »
Contribution of Trimeric Autotransporter C-Terminal Domains of Oligomeric Coiled-Coil Adhesin (Oca) Family Members YadA, UspA1, EibA, and Hia to Translocation of the YadA Passenger Domain and Virulence of Yersinia enterocolitica.
N. Ackermann, M. Tiller, G. Anding, A. Roggenkamp, and J. Heesemann (2008)
J. Bacteriol. 190, 5031-5043
   Abstract »    Full Text »    PDF »
Omp85Tt from Thermus thermophilus HB27: an Ancestral Type of the Omp85 Protein Family.
J. Nesper, A. Brosig, P. Ringler, G. J. Patel, S. A. Muller, J. H. Kleinschmidt, W. Boos, K. Diederichs, and W. Welte (2008)
J. Bacteriol. 190, 4568-4575
   Abstract »    Full Text »    PDF »
Highly Conserved Surface Proteins of Oral Spirochetes as Adhesins and Potent Inducers of Proinflammatory and Osteoclastogenic Factors.
H.-K. Jun, Y.-M. Kang, H.-R. Lee, S.-H. Lee, and B.-K. Choi (2008)
Infect. Immun. 76, 2428-2438
   Abstract »    Full Text »    PDF »
Opr86 Is Essential for Viability and Is a Potential Candidate for a Protective Antigen against Biofilm Formation by Pseudomonas aeruginosa.
Y. Tashiro, N. Nomura, R. Nakao, H. Senpuku, R. Kariyama, H. Kumon, S. Kosono, H. Watanabe, T. Nakajima, and H. Uchiyama (2008)
J. Bacteriol. 190, 3969-3978
   Abstract »    Full Text »    PDF »
Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli.
N. Ruiz, L. S. Gronenberg, D. Kahne, and T. J. Silhavy (2008)
PNAS 105, 5537-5542
   Abstract »    Full Text »    PDF »
Analysis of YfgL and YaeT Interactions through Bioinformatics, Mutagenesis, and Biochemistry.
P. Vuong, D. Bennion, J. Mantei, D. Frost, and R. Misra (2008)
J. Bacteriol. 190, 1507-1517
   Abstract »    Full Text »    PDF »
The Peripheral Membrane Subunits of the SAM Complex Function Codependently in Mitochondrial Outer Membrane Biogenesis.
N. C. Chan and T. Lithgow (2008)
Mol. Biol. Cell 19, 126-136
   Abstract »    Full Text »    PDF »
Alternative function for the mitochondrial SAM complex in biogenesis of {alpha}-helical TOM proteins.
D. Stojanovski, B. Guiard, V. Kozjak-Pavlovic, N. Pfanner, and C. Meisinger (2007)
J. Cell Biol. 179, 881-893
   Abstract »    Full Text »    PDF »
The Autodisplay Story, from Discovery to Biotechnical and Biomedical Applications.
J. Jose and T. F. Meyer (2007)
Microbiol. Mol. Biol. Rev. 71, 600-619
   Abstract »    Full Text »    PDF »
Cooperation of translocase complexes in mitochondrial protein import.
S. Kutik, B. Guiard, H. E. Meyer, N. Wiedemann, and N. Pfanner (2007)
J. Cell Biol. 179, 585-591
   Abstract »    Full Text »    PDF »
Additive and Synergistic Bactericidal Activity of Antibodies Directed against Minor Outer Membrane Proteins of Neisseria meningitidis.
V. E. Weynants, C. M. Feron, K. K. Goraj, M. P. Bos, P. A. Denoel, V. G. Verlant, J. Tommassen, I. R. A. Peak, R. C. Judd, M. P. Jennings, et al. (2007)
Infect. Immun. 75, 5434-5442
   Abstract »    Full Text »    PDF »
Identification of Novel Surface Proteins of Anaplasma phagocytophilum by Affinity Purification and Proteomics.
Y. Ge and Y. Rikihisa (2007)
J. Bacteriol. 189, 7819-7828
   Abstract »    Full Text »    PDF »
Short-Tailed Stx Phages Exploit the Conserved YaeT Protein To Disseminate Shiga Toxin Genes among Enterobacteria.
D. L. Smith, C. E. James, M. J. Sergeant, Y. Yaxian, J. R. Saunders, A. J. McCarthy, and H. E. Allison (2007)
J. Bacteriol. 189, 7223-7233
   Abstract »    Full Text »    PDF »
Alternative Splicing Gives Rise to Different Isoforms of the Neurospora crassa Tob55 Protein That Vary in Their Ability to Insert {beta}-Barrel Proteins Into the Outer Mitochondrial Membrane.
S. C. Hoppins, N. E. Go, A. Klein, S. Schmitt, W. Neupert, D. Rapaport, and F. E. Nargang (2007)
Genetics 177, 137-149
   Abstract »    Full Text »    PDF »
Structure of the Membrane Protein FhaC: A Member of the Omp85-TpsB Transporter Superfamily.
B. Clantin, A.-S. Delattre, P. Rucktooa, N. Saint, A. C. Meli, C. Locht, F. Jacob-Dubuisson, and V. Villeret (2007)
Science 317, 957-961
   Abstract »    Full Text »    PDF »
Structure and Function of an Essential Component of the Outer Membrane Protein Assembly Machine.
S. Kim, J. C. Malinverni, P. Sliz, T. J. Silhavy, S. C. Harrison, and D. Kahne (2007)
Science 317, 961-964
   Abstract »    Full Text »    PDF »
Surface-Exposed Proteins of Ehrlichia chaffeensis.
Y. Ge and Y. Rikihisa (2007)
Infect. Immun. 75, 3833-3841
   Abstract »    Full Text »    PDF »
Requirement for YaeT in the Outer Membrane Assembly of Autotransporter Proteins.
S. Jain and M. B. Goldberg (2007)
J. Bacteriol. 189, 5393-5398
   Abstract »    Full Text »    PDF »
Relaxed Cleavage Specificity of an Immunoglobulin A1 Protease from Neisseria meningitidis.
S. Vitovski and J. R. Sayers (2007)
Infect. Immun. 75, 2875-2885
   Abstract »    Full Text »    PDF »
Functional and Phylogenetic Properties of the Pore-forming beta-Barrel Transporters of the Omp85 Family.
R. Bredemeier, T. Schlegel, F. Ertel, A. Vojta, L. Borissenko, M. T. Bohnsack, M. Groll, A. von Haeseler, and E. Schleiff (2007)
J. Biol. Chem. 282, 1882-1890
   Abstract »    Full Text »    PDF »
The N-terminal domain of Tob55 has a receptor-like function in the biogenesis of mitochondrial {beta}-barrel proteins.
S. J. Habib, T. Waizenegger, A. Niewienda, S. A. Paschen, W. Neupert, and D. Rapaport (2007)
J. Cell Biol. 176, 77-88
   Abstract »    Full Text »    PDF »
Topology of the outer-membrane secretin PilQ from Neisseria meningitidis.
S. A. Frye, R. Assalkhou, R. F. Collins, R. C. Ford, C. Petersson, J. P. Derrick, and T. Tonjum (2006)
Microbiology 152, 3751-3764
   Abstract »    Full Text »    PDF »
Characterization of igaB, a Second Immunoglobulin A1 Protease Gene in Nontypeable Haemophilus influenzae..
M. M. Fernaays, A. J. Lesse, X. Cai, and T. F. Murphy (2006)
Infect. Immun. 74, 5860-5870
   Abstract »    Full Text »    PDF »
Differential Effects of yfgL Mutation on Escherichia coli Outer Membrane Proteins and Lipopolysaccharide..
E. S. Charlson, J. N. Werner, and R. Misra (2006)
J. Bacteriol. 188, 7186-7194
   Abstract »    Full Text »    PDF »
Role of the {alpha}-Helical Linker of the C-Terminal Translocator in the Biogenesis of the Serine Protease Subfamily of Autotransporters.
M. Kostakioti and C. Stathopoulos (2006)
Infect. Immun. 74, 4961-4969
   Abstract »    Full Text »    PDF »
Protein-translocating trimeric autotransporters of gram-negative bacteria..
D. S. H. Kim, Y. Chao, and M. H. Saier Jr (2006)
J. Bacteriol. 188, 5655-5667
   Full Text »    PDF »
Characterization of Pores Formed by YaeT (Omp85) from Escherichia coli.
J. F. Stegmeier and C. Andersen (2006)
J. Biochem. 140, 275-283
   Abstract »    Full Text »    PDF »
Polar Localization of the Autotransporter Family of Large Bacterial Virulence Proteins.
S. Jain, P. van Ulsen, I. Benz, M. A. Schmidt, R. Fernandez, J. Tommassen, and M. B. Goldberg (2006)
J. Bacteriol. 188, 4841-4850
   Abstract »    Full Text »    PDF »
The Periplasmic Folding of a Cysteineless Autotransporter Passenger Domain Interferes with Its Outer Membrane Translocation.
N. Rutherford, M.-E. Charbonneau, F. Berthiaume, J.-M. Betton, and M. Mourez (2006)
J. Bacteriol. 188, 4111-4116
   Abstract »    Full Text »    PDF »
Paired cysteine residues are required for high levels of the Helicobacter pylori autotransporter VacA..
D. P. Letley, J. L. Rhead, K. Bishop, and J. C. Atherton (2006)
Microbiology 152, 1319-1325
   Abstract »    Full Text »    PDF »
Domain architecture and structure of the bacterial cell division protein DivIB.
S. A. Robson and G. F. King (2006)
PNAS 103, 6700-6705
   Abstract »    Full Text »    PDF »
Channel Properties of TpsB Transporter FhaC Point to Two Functional Domains with a C-terminal Protein-conducting Pore.
A. C. Meli, H. Hodak, B. Clantin, C. Locht, G. Molle, F. Jacob-Dubuisson, and N. Saint (2006)
J. Biol. Chem. 281, 158-166
   Abstract »    Full Text »    PDF »
MsbA Is Not Required for Phospholipid Transport in Neisseria meningitidis.
B. Tefsen, M. P. Bos, F. Beckers, J. Tommassen, and H. de Cock (2005)
J. Biol. Chem. 280, 35961-35966
   Abstract »    Full Text »    PDF »
The Evolutionarily Related {beta}-Barrel Polypeptide Transporters from Pisum sativum and Nostoc PCC7120 Contain Two Distinct Functional Domains.
F. Ertel, O. Mirus, R. Bredemeier, S. Moslavac, T. Becker, and E. Schleiff (2005)
J. Biol. Chem. 280, 28281-28289
   Abstract »    Full Text »    PDF »
Loss of Outer Membrane Proteins without Inhibition of Lipid Export in an Escherichia coli YaeT Mutant.
W. T. Doerrler and C. R. H. Raetz (2005)
J. Biol. Chem. 280, 27679-27687
   Abstract »    Full Text »    PDF »
Mechanisms of Protein Export across the Bacterial Outer Membrane.
M. Kostakioti, C. L. Newman, D. G. Thanassi, and C. Stathopoulos (2005)
J. Bacteriol. 187, 4306-4314
   Full Text »    PDF »
Characterization of Six Lipoproteins in the {sigma}E Regulon.
C. Onufryk, M.-L. Crouch, F. C. Fang, and C. A. Gross (2005)
J. Bacteriol. 187, 4552-4561
   Abstract »    Full Text »    PDF »
All subtypes of the cytotoxin VacA adsorb to the surface of Helicobacter pylori post-secretion.
N. Fitchen, D. P Letley, P. O'Shea, J. C Atherton, P. Williams, and K. R Hardie (2005)
J. Med. Microbiol. 54, 621-630
   Abstract »    Full Text »    PDF »
Review: Lipopolysaccharide inner core oligosaccharide structure and outer membrane stability in human pathogens belonging to the Enterobacteriaceae.
E. Frirdich and C. Whitfield (2005)
Innate Immunity 11, 133-144
   Abstract »    PDF »
Interaction with Type IV Pili Induces Structural Changes in the Bacterial Outer Membrane Secretin PilQ.
R. F. Collins, S. A. Frye, S. Balasingham, R. C. Ford, T. Tonjum, and J. P. Derrick (2005)
J. Biol. Chem. 280, 18923-18930
   Abstract »    Full Text »    PDF »
Function of Neisserial Outer Membrane Phospholipase A in Autolysis and Assessment of Its Vaccine Potential.
M. P. Bos, B. Tefsen, P. Voet, V. Weynants, J. P. M. van Putten, and J. Tommassen (2005)
Infect. Immun. 73, 2222-2231
   Abstract »    Full Text »    PDF »
Type V Protein Secretion Pathway: the Autotransporter Story.
I. R. Henderson, F. Navarro-Garcia, M. Desvaux, R. C. Fernandez, and D. Ala'Aldeen (2004)
Microbiol. Mol. Biol. Rev. 68, 692-744
   Abstract »    Full Text »    PDF »
Evidence for conservation of architecture and physical properties of Omp85-like proteins throughout evolution.
N. K. Surana, S. Grass, G. G. Hardy, H. Li, D. G. Thanassi, and J. W. St. Geme III (2004)
PNAS 101, 14497-14502
   Abstract »    Full Text »    PDF »
Structure of the Neisseria meningitidis Outer Membrane PilQ Secretin Complex at 12 A Resolution.
R. F. Collins, S. A. Frye, A. Kitmitto, R. C. Ford, T. Tonjum, and J. P. Derrick (2004)
J. Biol. Chem. 279, 39750-39756
   Abstract »    Full Text »    PDF »
Role of the Pilot Protein YscW in the Biogenesis of the YscC Secretin in Yersinia enterocolitica.
P. Burghout, F. Beckers, E. de Wit, R. van Boxtel, G. R. Cornelis, J. Tommassen, and M. Koster (2004)
J. Bacteriol. 186, 5366-5375
   Abstract »    Full Text »    PDF »
Import Pathways of Chloroplast Interior Proteins and the Outer-Membrane Protein OEP14 Converge at Toc75.
S.-L. Tu, L.-J. Chen, M. D. Smith, Y.-s. Su, D. J. Schnell, and H.-m. Li (2004)
PLANT CELL 16, 2078-2088
   Abstract »    Full Text »    PDF »
Hydrophobic Residues of the Autotransporter EspP Linker Domain Are Important for Outer Membrane Translocation of Its Passenger.
J. J. Velarde and J. P. Nataro (2004)
J. Biol. Chem. 279, 31495-31504
   Abstract »    Full Text »    PDF »
Three Homologues, Including Two Membrane-bound Proteins, of the Disulfide Oxidoreductase DsbA in Neisseria meningitidis: EFFECTS ON BACTERIAL GROWTH AND BIOGENESIS OF FUNCTIONAL TYPE IV PILI.
C. R. Tinsley, R. Voulhoux, J.-L. Beretti, J. Tommassen, and X. Nassif (2004)
J. Biol. Chem. 279, 27078-27087
   Abstract »    Full Text »    PDF »
Identification of an outer membrane protein required for the transport of lipopolysaccharide to the bacterial cell surface.
M. P. Bos, B. Tefsen, J. Geurtsen, and J. Tommassen (2004)
PNAS 101, 9417-9422
   Abstract »    Full Text »    PDF »
Isolation of Outer Membrane of Synechocystis sp. PCC 6803 and Its Proteomic Characterization.
F. Huang, E. Hedman, C. Funk, T. Kieselbach, W. P. Schroder, and B. Norling (2004)
Mol. Cell. Proteomics 3, 586-595
   Abstract »    Full Text »    PDF »
Biogenesis of the Protein Import Channel Tom40 of the Mitochondrial Outer Membrane: INTERMEMBRANE SPACE COMPONENTS ARE INVOLVED IN AN EARLY STAGE OF THE ASSEMBLY PATHWAY.
N. Wiedemann, K. N. Truscott, S. Pfannschmidt, B. Guiard, C. Meisinger, and N. Pfanner (2004)
J. Biol. Chem. 279, 18188-18194
   Abstract »    Full Text »    PDF »
Ancient Invasions: From Endosymbionts to Organelles.
S. D. Dyall, M. T. Brown, and P. J. Johnson (2004)
Science 304, 253-257
   Abstract »    Full Text »    PDF »
The Protein Import Machinery of Mitochondria.
N. Wiedemann, A. E. Frazier, and N. Pfanner (2004)
J. Biol. Chem. 279, 14473-14476
   Full Text »    PDF »
The Omp85 family of proteins is essential for outer membrane biogenesis in mitochondria and bacteria.
I. Gentle, K. Gabriel, P. Beech, R. Waller, and T. Lithgow (2004)
J. Cell Biol. 164, 19-24
   Abstract »    Full Text »    PDF »
Sequence-specific Dimerization of the Transmembrane Domain of the "BH3-only" Protein BNIP3 in Membranes and Detergent.
E. S. Sulistijo, T. M. Jaszewski, and K. R. MacKenzie (2003)
J. Biol. Chem. 278, 51950-51956
   Abstract »    Full Text »    PDF »
An Essential Role of Sam50 in the Protein Sorting and Assembly Machinery of the Mitochondrial Outer Membrane.
V. Kozjak, N. Wiedemann, D. Milenkovic, C. Lohaus, H. E. Meyer, B. Guiard, C. Meisinger, and N. Pfanner (2003)
J. Biol. Chem. 278, 48520-48523
   Abstract »    Full Text »    PDF »
Molecular Basis of Bacterial Outer Membrane Permeability Revisited.
H. Nikaido (2003)
Microbiol. Mol. Biol. Rev. 67, 593-656
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)