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CREM-Dependent Transcription in Male Germ Cells Controlled by a Kinesin
Betina Macho,1Stefano Brancorsini,1Gian Maria Fimia,1Mitsutoshi Setou,2Nobutaka Hirokawa,2Paolo Sassone-Corsi1*
ACT is a LIM-only protein expressed exclusively in round
spermatids, where it cooperates with transcriptional activator CREMin
regulating various postmeiotic genes. Targeted inactivationof CREM
leads to a complete block of mouse spermiogenesis. Wesought to
identify the regulatory steps controlling the functionalinterplay
between CREM and ACT. We found that ACT selectivelyassociates with
KIF17b, a kinesin highly expressed in male germcells. The ACT-KIF17b
interaction is restricted to specific stagesof spermatogenesis and
directly determines the intracellular localizationof ACT. Sensitivity
to leptomycin B indicates that KIF17b canbe actively exported from the
nucleus through the Crm1 receptor.Thus, a kinesin directly controls
the activity of a transcriptionalcoactivator by a tight regulation of
its intracellular localization.
1 Institut de Génétique et de
Biologie Moléculaire et Cellulaire, B. P. 10142, 67404 Illkirch, Strasbourg, France.
2 Department of Cell
Biology and Anatomy, Graduate School of Medicine, University of Tokyo,
Hongo, 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
*
To whom correspondence should be addressed. E-mail:
paolosc{at}igbmc.u-strasbg.fr
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