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Science 13 December 2002:
Vol. 298. no. 5601, pp. 2188 - 2190
DOI: 10.1126/science.1077857
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Reports

Heart Regeneration in Zebrafish

Kenneth D. Poss,* Lindsay G. Wilson, Mark T. Keating*

Cardiac injury in mammals and amphibians typically leads to scarring, with minimal regeneration of heart muscle. Here, we demonstrate histologically that zebrafish fully regenerate hearts within 2 months of 20% ventricular resection. Regeneration occurs through robust proliferation of cardiomyocytes localized at the leading epicardial edge of the new myocardium. The hearts of zebrafish with mutations in the Mps1 mitotic checkpoint kinase, a critical cell cycle regulator, failed to regenerate and formed scars. Thus, injury-induced cardiomyocyte proliferation in zebrafish can overcome scar formation, allowing cardiac muscle regeneration. These findings indicate that zebrafish will be useful for genetically dissecting the molecular mechanisms of cardiac regeneration.

Department of Cell Biology, Department of Cardiology, Howard Hughes Medical Institute, Harvard Medical School, Children's Hospital, 320 Longwood Avenue, Boston, MA 02115, USA.
*   To whom correspondence should be addressed. E-mail: kposs{at}enders.tch.harvard.edu(K.D.P.); mkeating{at}enders.tch.harvard.edu (M.T.K.)

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