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Science 29 November 2002:
Vol. 298. no. 5599, pp. 1788 - 1790
DOI: 10.1126/science.1077123
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Reports

Molecular Hydrogen as an Energy Source for Helicobacter pylori

Jonathan W. Olson,* Robert J. Maierdagger

The gastric pathogen Helicobacter pylori is known to be able to use molecular hydrogen as a respiratory substrate when grown in the laboratory. We found that hydrogen is available in the gastric mucosa of mice and that its use greatly increased the stomach colonization by H. pylori. Hydrogenase activity in H. pylori is constitutive but increased fivefold upon incubation with hydrogen. Hydrogen concentrations measured in the stomachs of live mice were found to be 10 to 50 times as high as the H. pylori affinity for hydrogen. A hydrogenase mutant strain is much less efficient in its colonization of mice. Therefore, hydrogen present in animals as a consequence of normal colonic flora is an energy-yielding substrate that can facilitate the maintenance of a pathogenic bacterium.

Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
*   Present address: Department of Microbiology, North Carolina State University, Raleigh, NC 27695, USA.

dagger    To whom correspondence should be addressed. E-mail: rmaier{at}arches.uga.edu

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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J. Bacteriol. 190, 3244-3255
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Hydrogen and Nickel Metabolism in Helicobacter Species.
S. L. BENOIT and R. J. MAIER (2008)
Ann. N.Y. Acad. Sci. 1125, 242-251
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Nickel enzyme maturation in Helicobacter hepaticus: roles of accessory proteins in hydrogenase and urease activities.
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Differential expression of NiFe uptake-type hydrogenase genes in Salmonella enterica serovar Typhimurium.
A. L. Zbell, S. L. Benoit, and R. J. Maier (2007)
Microbiology 153, 3508-3516
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Function of Periplasmic Hydrogenases in the Sulfate-Reducing Bacterium Desulfovibrio vulgaris Hildenborough.
S. M. Caffrey, H.-S. Park, J. K. Voordouw, Z. He, J. Zhou, and G. Voordouw (2007)
J. Bacteriol. 189, 6159-6167
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Deep-sea vent {varepsilon}-proteobacterial genomes provide insights into emergence of pathogens.
S. Nakagawa, Y. Takaki, S. Shimamura, A.-L. Reysenbach, K. Takai, and K. Horikoshi (2007)
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J Dairy Sci 90, 2630-2640
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Interaction between the Helicobacter pylori accessory proteins HypA and UreE is needed for urease maturation.
S. L. Benoit, N. Mehta, M. V. Weinberg, C. Maier, and R. J. Maier (2007)
Microbiology 153, 1474-1482
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Infect. Immun. 74, 6821-6828
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Helicobacter pylori HP1512 Is a Nickel-Responsive NikR-Regulated Outer Membrane Protein.
G. S. Davis, E. L. Flannery, and H. L. T. Mobley (2006)
Infect. Immun. 74, 6811-6820
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Methionine Sulfoxide Reductase in Helicobacter pylori: Interaction with Methionine-Rich Proteins and Stress-Induced Expression..
P. Alamuri and R. J. Maier (2006)
J. Bacteriol. 188, 5839-5850
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Infect. Immun. 74, 3845-3852
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P. Alamuri, N. Mehta, A. Burk, and R. J. Maier (2006)
J. Bacteriol. 188, 5325-5330
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Pretreatment with Lactobacillus- and Bifidobacterium-containing yogurt can improve the efficacy of quadruple therapy in eradicating residual Helicobacter pylori infection after failed triple therapy..
B.-S. Sheu, H.-C. Cheng, A.-W. Kao, S.-T. Wang, Y.-J. Yang, H.-B. Yang, and J.-J. Wu (2006)
Am. J. Clinical Nutrition 83, 864-869
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The Nickel-Responsive Regulator NikR Controls Activation and Repression of Gene Transcription in Helicobacter pylori.
F. D. Ernst, E. J. Kuipers, A. Heijens, R. Sarwari, J. Stoof, C. W. Penn, J. G. Kusters, and A. H. M. van Vliet (2005)
Infect. Immun. 73, 7252-7258
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Complex Transcriptional Control Links NikABCDE-Dependent Nickel Transport with Hydrogenase Expression in Escherichia coli.
J. L. Rowe, G. L. Starnes, and P. T. Chivers (2005)
J. Bacteriol. 187, 6317-6323
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Helicobacter hepaticus Hydrogenase Mutants Are Deficient in Hydrogen-Supported Amino Acid Uptake and in Causing Liver Lesions in A/J Mice.
N. S. Mehta, S. Benoit, J. V. Mysore, R. S. Sousa, and R. J. Maier (2005)
Infect. Immun. 73, 5311-5318
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Iron-Responsive Regulation of the Helicobacter pylori Iron-Cofactored Superoxide Dismutase SodB Is Mediated by Fur.
F. D. Ernst, G. Homuth, J. Stoof, U. Mader, B. Waidner, E. J. Kuipers, M. Kist, J. G. Kusters, S. Bereswill, and A. H. M. van Vliet (2005)
J. Bacteriol. 187, 3687-3692
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Can the amount of Helicobacter pylori in the stomach be kept low through probiotic intake?.
M. Gotteland, S. Cruchet, and O. Brunser (2005)
Am. J. Clinical Nutrition 81, 939
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Reply to M Gotteland et al.
Y.-C. Su, W.-M. Wang, and K.-Y. Wang (2005)
Am. J. Clinical Nutrition 81, 939-940
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Respiratory Hydrogen Use by Salmonella enterica Serovar Typhimurium Is Essential for Virulence.
R. J. Maier, A. Olczak, S. Maier, S. Soni, and J. Gunn (2004)
Infect. Immun. 72, 6294-6299
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Nutrient Shock and Incubation Atmosphere Influence Recovery of Culturable Helicobacter pylori from Water.
N. F. Azevedo, A. P. Pacheco, C. W. Keevil, and M. J. Vieira (2004)
Appl. Envir. Microbiol. 70, 490-493
   Abstract »    Full Text »    PDF »
L-Serine, D- and L-proline and alanine as respiratory substrates of Helicobacter pylori: correlation between in vitro and in vivo amino acid levels.
K. Nagata, Y. Nagata, T. Sato, M. A. Fujino, K. Nakajima, and T. Tamura (2003)
Microbiology 149, 2023-2030
   Abstract »    Full Text »    PDF »
Hydrogen-Oxidizing Capabilities of Helicobacter hepaticus and In Vivo Availability of the Substrate.
R. J. Maier, J. Olson, and A. Olczak (2003)
J. Bacteriol. 185, 2680-2682
   Abstract »    Full Text »    PDF »
Characterization of Helicobacter pylori Nickel Metabolism Accessory Proteins Needed for Maturation of both Urease and Hydrogenase.
N. Mehta, J. W. Olson, and R. J. Maier (2003)
J. Bacteriol. 185, 726-734
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Flatus: An Energy Bonanza for Helicobacter pylori?.
(2002)
Journal Watch Infectious Diseases 2002, 14
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Science. ISSN 0036-8075 (print), 1095-9203 (online)