Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 29 November 2002:
Vol. 298. no. 5599, pp. 1788 - 1790
DOI: 10.1126/science.1077123
Prev | Table of Contents | Next

Reports

Molecular Hydrogen as an Energy Source for Helicobacter pylori

Jonathan W. Olson,* Robert J. Maierdagger

The gastric pathogen Helicobacter pylori is known to be able to use molecular hydrogen as a respiratory substrate when grown in the laboratory. We found that hydrogen is available in the gastric mucosa of mice and that its use greatly increased the stomach colonization by H. pylori. Hydrogenase activity in H. pylori is constitutive but increased fivefold upon incubation with hydrogen. Hydrogen concentrations measured in the stomachs of live mice were found to be 10 to 50 times as high as the H. pylori affinity for hydrogen. A hydrogenase mutant strain is much less efficient in its colonization of mice. Therefore, hydrogen present in animals as a consequence of normal colonic flora is an energy-yielding substrate that can facilitate the maintenance of a pathogenic bacterium.

Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
*   Present address: Department of Microbiology, North Carolina State University, Raleigh, NC 27695, USA.

dagger    To whom correspondence should be addressed. E-mail: rmaier{at}arches.uga.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
An Intact Urease Assembly Pathway Is Required To Compete with NikR for Nickel Ions in Helicobacter pylori.
E. L. Benanti and P. T. Chivers (2009)
J. Bacteriol. 191, 2405-2408
   Abstract »    Full Text »    PDF »
In Vivo Interactome of Helicobacter pylori Urease Revealed by Tandem Affinity Purification.
K. Stingl, K. Schauer, C. Ecobichon, A. Labigne, P. Lenormand, J.-C. Rousselle, A. Namane, and H. de Reuse (2008)
Mol. Cell. Proteomics 7, 2429-2441
   Abstract »    Full Text »    PDF »
Salmonella enterica Serovar Typhimurium NiFe Uptake-Type Hydrogenases Are Differentially Expressed In Vivo.
A. L. Zbell, S. E. Maier, and R. J. Maier (2008)
Infect. Immun. 76, 4445-4454
   Abstract »    Full Text »    PDF »
A Histidine-rich and Cysteine-rich Metal-binding Domain at the C Terminus of Heat Shock Protein A from Helicobacter pylori: IMPLICATION FOR NICKEL HOMEOSTASIS AND BISMUTH SUSCEPTIBILITY.
S. Cun, H. Li, R. Ge, M. C. M. Lin, and H. Sun (2008)
J. Biol. Chem. 283, 15142-15151
   Abstract »    Full Text »    PDF »
Functional Characterization and Mutagenesis of the Proposed Behavioral Sensor TlpD of Helicobacter pylori.
T. Schweinitzer, T. Mizote, N. Ishikawa, A. Dudnik, S. Inatsu, S. Schreiber, S. Suerbaum, S.-I. Aizawa, and C. Josenhans (2008)
J. Bacteriol. 190, 3244-3255
   Abstract »    Full Text »    PDF »
Nickel enzyme maturation in Helicobacter hepaticus: roles of accessory proteins in hydrogenase and urease activities.
S. L. Benoit, A. L. Zbell, and R. J. Maier (2007)
Microbiology 153, 3748-3756
   Abstract »    Full Text »    PDF »
Differential expression of NiFe uptake-type hydrogenase genes in Salmonella enterica serovar Typhimurium.
A. L. Zbell, S. L. Benoit, and R. J. Maier (2007)
Microbiology 153, 3508-3516
   Abstract »    Full Text »    PDF »
Function of Periplasmic Hydrogenases in the Sulfate-Reducing Bacterium Desulfovibrio vulgaris Hildenborough.
S. M. Caffrey, H.-S. Park, J. K. Voordouw, Z. He, J. Zhou, and G. Voordouw (2007)
J. Bacteriol. 189, 6159-6167
   Abstract »    Full Text »    PDF »
Deep-sea vent {varepsilon}-proteobacterial genomes provide insights into emergence of pathogens.
S. Nakagawa, Y. Takaki, S. Shimamura, A.-L. Reysenbach, K. Takai, and K. Horikoshi (2007)
PNAS 104, 12146-12150
   Abstract »    Full Text »    PDF »
Effect of Bifidobacterium bifidum Fermented Milk on Helicobacter pylori and Serum Pepsinogen Levels in Humans.
K. Miki, Y. Urita, F. Ishikawa, T. Iino, H. Shibahara-Sone, R. Akahoshi, S. Mizusawa, A. Nose, D. Nozaki, K. Hirano, et al. (2007)
J Dairy Sci 90, 2630-2640
   Abstract »    Full Text »    PDF »
Interaction between the Helicobacter pylori accessory proteins HypA and UreE is needed for urease maturation.
S. L. Benoit, N. Mehta, M. V. Weinberg, C. Maier, and R. J. Maier (2007)
Microbiology 153, 1474-1482
   Abstract »    Full Text »    PDF »
NikR Mediates Nickel-Responsive Transcriptional Repression of the Helicobacter pylori Outer Membrane Proteins FecA3 (HP1400) and FrpB4 (HP1512).
F. D. Ernst, J. Stoof, W. M. Horrevoets, E. J. Kuipers, J. G. Kusters, and A. H. M. van Vliet (2006)
Infect. Immun. 74, 6821-6828
   Abstract »    Full Text »    PDF »
Helicobacter pylori HP1512 Is a Nickel-Responsive NikR-Regulated Outer Membrane Protein.
G. S. Davis, E. L. Flannery, and H. L. T. Mobley (2006)
Infect. Immun. 74, 6811-6820
   Abstract »    Full Text »    PDF »
Methionine Sulfoxide Reductase in Helicobacter pylori: Interaction with Methionine-Rich Proteins and Stress-Induced Expression..
P. Alamuri and R. J. Maier (2006)
J. Bacteriol. 188, 5839-5850
   Abstract »    Full Text »    PDF »
The Novel Helicobacter pylori CznABC Metal Efflux Pump Is Required for Cadmium, Zinc, and Nickel Resistance, Urease Modulation, and Gastric Colonization.
F. N. Stahler, S. Odenbreit, R. Haas, J. Wilrich, A. H. M. V. Vliet, J. G. Kusters, M. Kist, and S. Bereswill (2006)
Infect. Immun. 74, 3845-3852
   Abstract »    Full Text »    PDF »
Regulation of the Helicobacter pylori Fe-S Cluster Synthesis Protein NifS by Iron, Oxidative Stress Conditions, and Fur..
P. Alamuri, N. Mehta, A. Burk, and R. J. Maier (2006)
J. Bacteriol. 188, 5325-5330
   Abstract »    Full Text »    PDF »
Pretreatment with Lactobacillus- and Bifidobacterium-containing yogurt can improve the efficacy of quadruple therapy in eradicating residual Helicobacter pylori infection after failed triple therapy..
B.-S. Sheu, H.-C. Cheng, A.-W. Kao, S.-T. Wang, Y.-J. Yang, H.-B. Yang, and J.-J. Wu (2006)
Am. J. Clinical Nutrition 83, 864-869
   Abstract »    Full Text »    PDF »
The Nickel-Responsive Regulator NikR Controls Activation and Repression of Gene Transcription in Helicobacter pylori.
F. D. Ernst, E. J. Kuipers, A. Heijens, R. Sarwari, J. Stoof, C. W. Penn, J. G. Kusters, and A. H. M. van Vliet (2005)
Infect. Immun. 73, 7252-7258
   Abstract »    Full Text »    PDF »
Complex Transcriptional Control Links NikABCDE-Dependent Nickel Transport with Hydrogenase Expression in Escherichia coli.
J. L. Rowe, G. L. Starnes, and P. T. Chivers (2005)
J. Bacteriol. 187, 6317-6323
   Abstract »    Full Text »    PDF »
Helicobacter hepaticus Hydrogenase Mutants Are Deficient in Hydrogen-Supported Amino Acid Uptake and in Causing Liver Lesions in A/J Mice.
N. S. Mehta, S. Benoit, J. V. Mysore, R. S. Sousa, and R. J. Maier (2005)
Infect. Immun. 73, 5311-5318
   Abstract »    Full Text »    PDF »
Iron-Responsive Regulation of the Helicobacter pylori Iron-Cofactored Superoxide Dismutase SodB Is Mediated by Fur.
F. D. Ernst, G. Homuth, J. Stoof, U. Mader, B. Waidner, E. J. Kuipers, M. Kist, J. G. Kusters, S. Bereswill, and A. H. M. van Vliet (2005)
J. Bacteriol. 187, 3687-3692
   Abstract »    Full Text »    PDF »
Can the amount of Helicobacter pylori in the stomach be kept low through probiotic intake?.
M. Gotteland, S. Cruchet, and O. Brunser (2005)
Am. J. Clinical Nutrition 81, 939
   Full Text »    PDF »
Reply to M Gotteland et al.
Y.-C. Su, W.-M. Wang, and K.-Y. Wang (2005)
Am. J. Clinical Nutrition 81, 939-940
   Full Text »    PDF »
Respiratory Hydrogen Use by Salmonella enterica Serovar Typhimurium Is Essential for Virulence.
R. J. Maier, A. Olczak, S. Maier, S. Soni, and J. Gunn (2004)
Infect. Immun. 72, 6294-6299
   Abstract »    Full Text »    PDF »
Nutrient Shock and Incubation Atmosphere Influence Recovery of Culturable Helicobacter pylori from Water.
N. F. Azevedo, A. P. Pacheco, C. W. Keevil, and M. J. Vieira (2004)
Appl. Envir. Microbiol. 70, 490-493
   Abstract »    Full Text »    PDF »
L-Serine, D- and L-proline and alanine as respiratory substrates of Helicobacter pylori: correlation between in vitro and in vivo amino acid levels.
K. Nagata, Y. Nagata, T. Sato, M. A. Fujino, K. Nakajima, and T. Tamura (2003)
Microbiology 149, 2023-2030
   Abstract »    Full Text »    PDF »
Hydrogen-Oxidizing Capabilities of Helicobacter hepaticus and In Vivo Availability of the Substrate.
R. J. Maier, J. Olson, and A. Olczak (2003)
J. Bacteriol. 185, 2680-2682
   Abstract »    Full Text »    PDF »
Characterization of Helicobacter pylori Nickel Metabolism Accessory Proteins Needed for Maturation of both Urease and Hydrogenase.
N. Mehta, J. W. Olson, and R. J. Maier (2003)
J. Bacteriol. 185, 726-734
   Abstract »    Full Text »    PDF »
Flatus: An Energy Bonanza for Helicobacter pylori?.
(2002)
Journal Watch Infectious Diseases 2002, 14
   Full Text »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)