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Science 1 November 2002:
Vol. 298. no. 5595, pp. 1036 - 1039
DOI: 10.1126/science.1076327
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Reports
RNA Polymerase II Transcription in Murine Cells Lacking the TATA Binding Protein
Igor Martianov,
Stephane Viville,
Irwin Davidson*
Inactivation of the murine TATA binding protein
(TBP) gene by homologous recombination leads to growth arrest
and apoptosis at the embryonic blastocyst stage. However, after loss of
TBP, RNA polymerase II (pol II) remains in a transcriptionally
active phosphorylation state, and in situ run-on
experiments showed high levels of pol II transcription comparable to
those of wild-type cells. In contrast, pol I and pol III transcription
was arrested. Our results show a differential dependency of the RNA
polymerases on TBP and provide evidence for TBP-independent pol II
transcriptional mechanisms that allow reinitiation and maintenance of
gene transcription in vivo.
Institut de Génétique et de Biologie Moléculaire
et Cellulaire (IGBMC), CNRS/INSERM/ULP, B.P. 163, 67404 Illkirch
Cédex, Communauté Urbaine de Strasbourg, France
*
To whom correspondence should be addressed. E-mail:
irwin{at}titus.u-strasbg.fr
Read the Full Text
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