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Coordinate Regulation of Transcription and Splicing by Steroid Receptor Coregulators
Didier Auboeuf,1Arnd Hönig,2*Susan M. Berget,2Bert W. O'Malley1
Recent observations indicating that promoter identity influences
alternative RNA-processing decisions have created interestin the
regulatory interactions between RNA polymerase II transcriptionand
precursor messenger RNA (pre-mRNA) processing. We examinedthe
impact of steroid receptor-mediated transcription on RNA processingwith reporter genes subject to alternative splicing driven bysteroid-sensitive promoters. Steroid hormones affected the processingof pre-mRNA synthesized from steroid-sensitive promoters, butnot from
steroid-unresponsive promoters, in a steroid receptor-dependentand
receptor-selective manner. Several nuclear receptor coregulatorsshowed
differential splicing effects, suggesting that steroidhormone
receptors may simultaneously control gene transcriptionactivity and
exon content of the product mRNA by recruiting coregulatorsinvolved in
both processes.
1 Department of Molecular and Cellular Biology,
2 Department of Biochemistry and Molecular Biology,
Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
*
Present address: Department of Obstetrics and Gynecology,
University of Tubingen, 72076 Tubingen, Germany.
To whom correspondence should be addressed. E-mail:
berto{at}bcm.tmc.edu
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