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Originally published in Science Express on 8 August 2002
Science 11 October 2002:
Vol. 298. no. 5592, pp. 406 - 409
DOI: 10.1126/science.1075119
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Reports

Role of ANC-1 in Tethering Nuclei to the Actin Cytoskeleton

Daniel A. Starr, Min Han*

Mutations in anc-1 (nuclear anchorage defective) disrupt the positioning of nuclei and mitochondria in Caenorhabditis elegans. ANC-1 is shown to consist of mostly coiled regions with a nuclear envelope localization domain (called the KASH domain) and an actin-binding domain; this structure was conserved with the Drosophila protein Msp-300 and the mammalian Syne proteins. Antibodies against ANC-1 localized cytoplasmically and were enriched at the nuclear periphery in an UNC-84-dependent manner. Overexpression of the KASH domain or the actin-binding domain caused a dominant negative anchorage defect. Thus, ANC-1 may connect nuclei to the cytoskeleton by interacting with UNC-84 at the nuclear envelope and with actin in the cytoplasm.

Howard Hughes Medical Institute and Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.
*   To whom correspondence should be addressed. E-mail: mhan{at}colorado.edu

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