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Science 27 September 2002:
Vol. 297. no. 5590, pp. 2253 - 2256
DOI: 10.1126/science.1074170
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Reports

A Single P450 Allele Associated with Insecticide Resistance in Drosophila

P. J. Daborn,1 J. L. Yen,1 M. R. Bogwitz,2 G. Le Goff,1 E. Feil,1 S. Jeffers,3 N. Tijet,4 T. Perry,2 D. Heckel,2 P. Batterham,2 R. Feyereisen,5 T. G. Wilson,3 R. H. ffrench-Constant1*

Insecticide resistance is one of the most widespread genetic changes caused by human activity, but we still understand little about the origins and spread of resistant alleles in global populations of insects. Here, via microarray analysis of all P450s in Drosophila melanogaster, we show that DDT-R, a gene conferring resistance to DDT, is associated with overtranscription of a single cytochrome P450 gene, Cyp6g1. Transgenic analysis of Cyp6g1 shows that overtranscription of this gene alone is both necessary and sufficient for resistance. Resistance and up-regulation in Drosophila populations are associated with a single Cyp6g1 allele that has spread globally. This allele is characterized by the insertion of an Accord transposable element into the 5' end of the Cyp6g1 gene.

1 Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
2 Centre for Environmental Stress and Adaptation Research (CESAR), Department of Genetics, University of Melbourne, Victoria, 3052, Australia.
3 Department of Biology, Colorado State University, Fort Collins, CO 80523, USA.
4 Department of Entomology, University of Arizona, Tucson, AZ 85721, USA.
5 INRA Centre de Recherches d'Antibes, 1382 Route de Biot, 06560, Valbonne, France.
*   To whom correspondence should be addressed. E-mail: bssrfc{at}bath.ac.uk

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Science. ISSN 0036-8075 (print), 1095-9203 (online)