Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 23 August 2002:
Vol. 297. no. 5585, pp. 1345 - 1348
DOI: 10.1126/science.1072366
Prev | Table of Contents | Next

Reports

Distinct Modes of Signal Recognition Particle Interaction with the Ribosome

Martin R. Pool,1* Joachim Stumm,1 Tudor A. Fulga,2 Irmgard Sinning,2dagger Bernhard Dobberstein1

Signal recognition particle (SRP), together with its receptor (SR), mediates the targeting of ribosome-nascent chain complexes to the endoplasmic reticulum. Using protein cross-linking, we detected distinct modes in the binding of SRP to the ribosome. During signal peptide recognition, SRP54 is positioned at the exit site close to ribosomal proteins L23a and L35. When SRP54 contacts SR, SRP54 is rearranged such that it is no longer close to L23a. This repositioning may allow the translocon to dock with the ribosome, leading to insertion of the signal peptide into the translocation channel.

1 Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), D-69120 Heidelberg, Germany.
2 Structural Biology Programme, European Molecular Biology Laboratory (EMBL), D-69117 Heidelberg, Germany.
*   To whom correspondence should be addressed. E-mail: mrp{at}zmbh.uni-heidelberg.de

dagger    Present address: Biochemiezentrum der Universität Heidelberg (BZH), D-69120 Heidelberg, Germany.

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Eeyarestatin I inhibits Sec61-mediated protein translocation at the endoplasmic reticulum.
B. C. S. Cross, C. McKibbin, A. C. Callan, P. Roboti, M. Piacenti, C. Rabu, C. M. Wilson, R. Whitehead, S. L. Flitsch, M. R. Pool, et al. (2009)
J. Cell Sci. 122, 4393-4400
   Abstract »    Full Text »    PDF »
Distinct cytoplasmic maturation steps of 40S ribosomal subunit precursors require hRio2.
I. Zemp, T. Wild, M.-F. O'Donohue, F. Wandrey, B. Widmann, P.-E. Gleizes, and U. Kutay (2009)
J. Cell Biol. 185, 1167-1180
   Abstract »    Full Text »    PDF »
A trans-membrane segment inside the ribosome exit tunnel triggers RAMP4 recruitment to the Sec61p translocase.
M. R. Pool (2009)
J. Cell Biol. 185, 889-902
   Abstract »    Full Text »    PDF »
Multiple conformational switches in a GTPase complex control co-translational protein targeting.
X. Zhang, C. Schaffitzel, N. Ban, and S.-o. Shan (2009)
PNAS 106, 1754-1759
   Abstract »    Full Text »    PDF »
Ribosome-associated Complex Binds to Ribosomes in Close Proximity of Rpl31 at the Exit of the Polypeptide Tunnel in Yeast.
K. Peisker, D. Braun, T. Wolfle, J. Hentschel, U. Funfschilling, G. Fischer, A. Sickmann, and S. Rospert (2008)
Mol. Biol. Cell 19, 5279-5288
   Abstract »    Full Text »    PDF »
Access to Ribosomal Protein Rpl25p by the Signal Recognition Particle Is Required for Efficient Cotranslational Translocation.
J. A. Dalley, A. Selkirk, and M. R. Pool (2008)
Mol. Biol. Cell 19, 2876-2884
   Abstract »    Full Text »    PDF »
Distinct targeting pathways for the membrane insertion of tail-anchored (TA) proteins.
V. Favaloro, M. Spasic, B. Schwappach, and B. Dobberstein (2008)
J. Cell Sci. 121, 1832-1840
   Abstract »    Full Text »    PDF »
Arabidopsis Ribosomal Proteins RPL23aA and RPL23aB Are Differentially Targeted to the Nucleolus and Are Disparately Required for Normal Development.
R. F. Degenhardt and P. C. Bonham-Smith (2008)
Plant Physiology 147, 128-142
   Abstract »    Full Text »    PDF »
Down-Regulation of the Trypanosomatid Signal Recognition Particle Affects the Biogenesis of Polytopic Membrane Proteins but Not of Signal Peptide-Containing Proteins.
Y. Lustig, Y. Vagima, H. Goldshmidt, A. Erlanger, V. Ozeri, J. Vince, M. J. McConville, D. M. Dwyer, S. M. Landfear, and S. Michaeli (2007)
Eukaryot. Cell 6, 1865-1875
   Abstract »    Full Text »    PDF »
Interaction of signal-recognition particle 54 GTPase domain and signal-recognition particle RNA in the free signal-recognition particle.
T. Hainzl, S. Huang, and A. E. Sauer-Eriksson (2007)
PNAS 104, 14911-14916
   Abstract »    Full Text »    PDF »
Conformational changes in the GTPase modules of the signal reception particle and its receptor drive initiation of protein translocation.
S.-o. Shan, S. Chandrasekar, and P. Walter (2007)
J. Cell Biol. 178, 611-620
   Abstract »    Full Text »    PDF »
Functional conservation between structurally diverse ribosomal proteins from Drosophila melanogaster and Saccharomyces cerevisiae: fly L23a can substitute for yeast L25 in ribosome assembly and function.
C. L. N. Ross, R. R. Patel, T. C. Mendelson, and V. C. Ware (2007)
Nucleic Acids Res. 35, 4503-4514
   Abstract »    Full Text »    PDF »
Association of Protein Biogenesis Factors at the Yeast Ribosomal Tunnel Exit Is Affected by the Translational Status and Nascent Polypeptide Sequence.
U. Raue, S. Oellerer, and S. Rospert (2007)
J. Biol. Chem. 282, 7809-7816
   Abstract »    Full Text »    PDF »
Analysis of the Ribosomal Protein S19 Interactome.
S. Orru, A. Aspesi, M. Armiraglio, M. Caterino, F. Loreni, M. Ruoppolo, C. Santoro, and I. Dianzani (2007)
Mol. Cell. Proteomics 6, 382-393
   Abstract »    Full Text »    PDF »
Transcript profiles at different growth stages and tap-root zones identify correlated developmental and metabolic pathways of sugar beet.
D. Bellin, B. Schulz, T. R. Soerensen, F. Salamini, and K. Schneider (2007)
J. Exp. Bot. 58, 699-715
   Abstract »    Full Text »    PDF »
Ribosome Binding to and Dissociation from Translocation Sites of the Endoplasmic Reticulum Membrane.
J. Schaletzky and T. A. Rapoport (2006)
Mol. Biol. Cell 17, 3860-3869
   Abstract »    Full Text »    PDF »
Sequence-specific Interactions of Nascent Escherichia coli Polypeptides with Trigger Factor and Signal Recognition Particle.
R. S. Ullers, E. N. G. Houben, J. Brunner, B. Oudega, N. Harms, and J. Luirink (2006)
J. Biol. Chem. 281, 13999-14005
   Abstract »    Full Text »    PDF »
Nuclear Recycling of the Pre-60S Ribosomal Subunit-Associated Factor Arx1 Depends on Rei1 in Saccharomyces cerevisiae.
N.-J. Hung and A. W. Johnson (2006)
Mol. Cell. Biol. 26, 3718-3727
   Abstract »    Full Text »    PDF »
Signal recognition particle receptor exposes the ribosomal translocon binding site..
M. Halic, M. Gartmann, O. Schlenker, T. Mielke, M. R. Pool, I. Sinning, and R. Beckmann (2006)
Science 312, 745-747
   Abstract »    Full Text »    PDF »
Homodimerization of the G protein SRbeta in the nucleotide-free state involves proline cis/trans isomerization in the switch II region.
T. U. Schwartz, D. Schmidt, S. G. Brohawn, and G. Blobel (2006)
PNAS 103, 6823-6828
   Abstract »    Full Text »    PDF »
An Unusual Signal Peptide Extension Inhibits the Binding of Bacterial Presecretory Proteins to the Signal Recognition Particle, Trigger Factor, and the SecYEG Complex.
J. H. Peterson, R. L. Szabady, and H. D. Bernstein (2006)
J. Biol. Chem. 281, 9038-9048
   Abstract »    Full Text »    PDF »
Alternate Recruitment of Signal Recognition Particle and Trigger Factor to the Signal Sequence of a Growing Nascent Polypeptide.
G. Eisner, M. Moser, U. Schafer, K. Beck, and M. Muller (2006)
J. Biol. Chem. 281, 7172-7179
   Abstract »    Full Text »    PDF »
A Conserved Motif Is Prerequisite for the Interaction of NAC with Ribosomal Protein L23 and Nascent Chains.
R. D. Wegrzyn, D. Hofmann, F. Merz, R. Nikolay, T. Rauch, C. Graf, and E. Deuerling (2006)
J. Biol. Chem. 281, 2847-2857
   Abstract »    Full Text »    PDF »
Alu RNP and Alu RNA regulate translation initiation in vitro..
J. Hasler and K. Strub (2006)
Nucleic Acids Res. 34, 2374-2385
   Abstract »    Full Text »    PDF »
The Trypanosoma brucei signal recognition particle lacks the Alu-domain-binding proteins: purification and functional analysis of its binding proteins by RNAi.
Y. Lustig, H. Goldshmidt, S. Uliel, and S. Michaeli (2005)
J. Cell Sci. 118, 4551-4562
   Abstract »    Full Text »    PDF »
Conformation of 4.5S RNA in the signal recognition particle and on the 30S ribosomal subunit.
S.-Q. GU, J. JOCKEL, P. BEINKER, J. WARNECKE, Y. P. SEMENKOV, M. V. RODNINA, and W. WINTERMEYER (2005)
RNA 11, 1374-1384
   Abstract »    Full Text »    PDF »
Early encounters of a nascent membrane protein: specificity and timing of contacts inside and outside the ribosome.
E. N.G. Houben, R. Zarivach, B. Oudega, and J. Luirink (2005)
J. Cell Biol. 170, 27-35
   Abstract »    Full Text »    PDF »
Signal recognition particle assembly in relation to the function of amplified nucleoli of Xenopus oocytes.
J. Sommerville, C. L. Brumwell, J. C. R. Politz, and T. Pederson (2005)
J. Cell Sci. 118, 1299-1307
   Abstract »    Full Text »    PDF »
Differential Regulation of the TRAIL Death Receptors DR4 and DR5 by the Signal Recognition Particle.
Y.-G. Ren, K. W. Wagner, D. A. Knee, P. Aza-Blanc, M. Nasoff, and Q. L. Deveraux (2004)
Mol. Biol. Cell 15, 5064-5074
   Abstract »    Full Text »    PDF »
Saccharomyces SRP RNA secondary structures: A conserved S-domain and extended Alu-domain.
R. W. VAN NUES and J. D. BROWN (2004)
RNA 10, 75-89
   Abstract »    Full Text »    PDF »
Crystal structure of the complete core of archaeal signal recognition particle and implications for interdomain communication.
K. R. Rosendal, K. Wild, G. Montoya, and I. Sinning (2003)
PNAS 100, 14701-14706
   Abstract »    Full Text »    PDF »
Dual recognition of the ribosome and the signal recognition particle by the SRP receptor during protein targeting to the endoplasmic reticulum.
E. C. Mandon, Y. Jiang, and R. Gilmore (2003)
J. Cell Biol. 162, 575-585
   Abstract »    Full Text »    PDF »
Biogenesis and nuclear export of ribosomal subunits in higher eukaryotes depend on the CRM1 export pathway.
F. Thomas and U. Kutay (2003)
J. Cell Sci. 116, 2409-2419
   Abstract »    Full Text »    PDF »
Interplay of signal recognition particle and trigger factor at L23 near the nascent chain exit site on the Escherichia coli ribosome.
R. S. Ullers, E. N.G. Houben, A. Raine, C. M. ten Hagen-Jongman, M. Ehrenberg, J. Brunner, B. Oudega, N. Harms, and J. Luirink (2003)
J. Cell Biol. 161, 679-684
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)