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Science 16 August 2002:
Vol. 297. no. 5584, pp. 1193 - 1196
DOI: 10.1126/science.1071362
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Reports

Amphiphysin 2 (Bin1) and T-Tubule Biogenesis in Muscle

Eunkyung Lee,1* Melissa Marcucci,1* Laurie Daniell,1 Marc Pypaert,1 Ora A. Weisz,2 Gian-Carlo Ochoa,1 Khashayar Farsad,1 Markus R. Wenk,1 Pietro De Camilli1dagger

In striated muscle, the plasma membrane forms tubular invaginations (transverse tubules or T-tubules) that function in depolarization-contraction coupling. Caveolin-3 and amphiphysin were implicated in their biogenesis. Amphiphysin isoforms have a putative role in membrane deformation at endocytic sites. An isoform of amphiphysin 2 concentrated at T-tubules induced tubular plasma membrane invaginations when expressed in nonmuscle cells. This property required exon 10, a phosphoinositide-binding module. In developing myotubes, amphiphysin 2 and caveolin-3 segregated in tubular and vesicular portions of the T-tubule system, respectively. These findings support a role of the bilayer-deforming properties of amphiphysin at T-tubules and, more generally, a physiological role of amphiphysin in membrane deformation.

1 Department of Cell Biology and Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06510, USA.
2 Renal-Electrolyte Division, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, PA 15261, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: pietro.decamilli{at}yale.edu

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