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Originally published in Science Express on 6 June 2002
Science 26 July 2002:
Vol. 297. no. 5581, pp. 612 - 615
DOI: 10.1126/science.1072309
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Reports

Role of Formins in Actin Assembly: Nucleation and Barbed-End Association

David Pruyne,1* Marie Evangelista,2* Changsong Yang,3 Erfei Bi,4 Sally Zigmond,3 Anthony Bretscher,1dagger Charles Boone25dagger

Nucleation of branched actin filaments by the Arp2/3 complex is a conserved process in eukaryotic cells, yet the source of unbranched actin filaments has remained obscure. In yeast, formins stimulate assembly of actin cables independently of Arp2/3. Here, the conserved core of formin homology domains 1 and 2 of Bni1p (Bni1pFH1FH2) was found to nucleate unbranched actin filaments in vitro. Bni1pFH2 provided the minimal region sufficient for nucleation. Unique among actin nucleators, Bni1pFH1FH2 remained associated with the growing barbed ends of filaments. This combination of properties suggests a direct role for formins in regulating nucleation and polarization of unbranched filamentous actin structures.

1 Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
2 Department of Biology, Queen's University, Kingston, Ontario, K7L 3N6 Canada.
3 Biology Department, University of Pennsylvania, Philadelphia, PA 19104-6018, USA.
4 Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
5 Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5G 1L6 Canada.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: apb5{at}cornell.edu (A.B.), charlie.boone{at}utoronto.ca (C.B.)

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