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Originally published in Science Express on 27 June 2002
Science 19 July 2002:
Vol. 297. no. 5580, pp. 395 - 400
DOI: 10.1126/science.1070850
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Reports

Systematic Identification of Pathways That Couple Cell Growth and Division in Yeast

Paul Jorgensen,12* Joy L. Nishikawa,12* Bobby-Joe Breitkreutz,2 Mike Tyers12dagger

Size homeostasis in budding yeast requires that cells grow to a critical size before commitment to division in the late prereplicative growth phase of the cell cycle, an event termed Start. We determined cell size distributions for the complete set of ~6000 Saccharomyces cerevisiae gene deletion strains and identified ~500 abnormally small (whi) or large (lge) mutants. Genetic analysis revealed a complex network of newly found factors that govern critical cell size at Start, the most potent of which were Sfp1, Sch9, Cdh1, Prs3, and Whi5. Ribosome biogenesis is intimately linked to cell size through Sfp1, a transcription factor that controls the expression of at least 60 genes implicated in ribosome assembly. Cell growth and division appear to be coupled by multiple conserved mechanisms.

1 Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8.
2 Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: tyers{at}mshri.on.ca

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Does size matter?.
W. A. Wells (2002)
J. Cell Biol. 158, 1156-1159
   Abstract »    Full Text »    PDF »


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