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Comparative Genome Sequencing for Discovery of Novel Polymorphisms in Bacillus anthracis
Timothy D. Read,1Steven L. Salzberg,1Mihai Pop,1Martin Shumway,1Lowell Umayam,1Lingxia Jiang,1Erik Holtzapple,1Joseph D. Busch,2Kimothy L. Smith,2James M. Schupp,2Daniel Solomon,2Paul Keim,2Claire M. Fraser1*
Comparison of the whole-genome sequence of
Bacillus anthracis isolated from a victim of a recent
bioterrorist anthrax attackwith a reference reveals 60 new markers
that include single nucleotidepolymorphisms (SNPs), inserted or
deleted sequences, and tandemrepeats. Genome comparison detected four
high-quality SNPs betweenthe two sequenced B. anthracis
chromosomes and seven differencesamong different preparations of the
reference genome. These markershave been tested on a collection of
anthrax isolates and werefound to divide these samples into distinct
families. These resultsdemonstrate that genome-based analysis of
microbial pathogenswill provide a powerful new tool for investigation
of infectiousdisease outbreaks.
1 The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
2 Department of Biological Sciences, Northern
Arizona University, Flagstaff, AZ 86011, USA.
*
To whom correspondence should be addressed. E-mail:
anthrax{at}tigr.org
The editors suggest the following Related Resources on Science sites:
In Science Magazine
PERSPECTIVES
Craig A. Cummings and David A. Relman (14 June 2002) Science296 (5575), 1976.
[DOI: 10.1126/science.1073125] |Summary »|Full Text »|PDF »
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