Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Dynamics of Thymocyte-Stromal Cell Interactions Visualized by Two-Photon Microscopy
Philippe Bousso,1Nirav R. Bhakta,2*Richard S. Lewis,2Ellen Robey1
Thymocytes are selected to mature according to their ability to
interact with self major histocompatibility complex (MHC)-peptidecomplexes displayed on the thymic stroma. Using two-photon microscopy,we performed real-time analysis of the cellular contacts madeby
developing thymocytes undergoing positive selection in a
three-dimensionalthymic organ culture. A large fraction of thymocytes
within thesecultures were highly motile. MHC recognition was found to
increasethe duration of thymocyte-stromal cell interactions
and occurredas both long-lived cellular associations displaying stable
cell-cellcontacts and as shorter, highly dynamic contacts. Our resultsidentify the diversity and dynamics of thymocyte interactionsduring
positive selection.
1 Department of Molecular and Cell Biology,
University of California, Berkeley, CA 94720, USA.
2 Department of Molecular and Cellular Physiology,
Stanford University School of Medicine, Stanford, CA 94305, USA.
*
This author made independent, essential contributions to this
work.
To whom correspondence should be addressed. E-mail:
erobey{at}uclink4.berkeley.edu
The editors suggest the following Related Resources on Science sites:
In Science Magazine
REPORTS
Mark J. Miller, Sindy H. Wei, Ian Parker, and Michael D. Cahalan (7 June 2002) Science296 (5574), 1869.
[DOI: 10.1126/science.1070051] |Abstract »|Full Text »|PDF »|Supplemental Data »
PERSPECTIVES
Ulrich H. von Andrian (7 June 2002) Science296 (5574), 1815.
[DOI: 10.1126/science.296.5574.1815] |Summary »|Full Text »|PDF »
REPORTS
Sabine Stoll, Jérôme Delon, Tilmann M. Brotz, and Ronald N. Germain (7 June 2002) Science296 (5574), 1873.
[DOI: 10.1126/science.1071065] |Abstract »|Full Text »|PDF »|Supplemental Data »
G. Anderson and E. J. Jenkinson (2008)
J. Immunol.
181, 7435-7436
|Full Text »|PDF »
Thymocyte-Dendritic Cell Interactions near Sources of CCR7 Ligands in the Thymic Cortex.
E. Ladi, T. A. Schwickert, T. Chtanova, Y. Chen, P. Herzmark, X. Yin, H. Aaron, S. W. Chan, M. Lipp, B. Roysam, et al. (2008)
J. Immunol.
181, 7014-7023
|Abstract »|Full Text »|PDF »
How the thymus designs antigen-specific and self-tolerant T cell receptor sequences.
A. Kosmrlj, A. K. Jha, E. S. Huseby, M. Kardar, and A. K. Chakraborty (2008)
PNAS
105, 16671-16676
|Abstract »|Full Text »|PDF »
How Antigen Quantity and Quality Determine T-Cell Decisions in Lymphoid Tissue.
H. Zheng, B. Jin, S. E. Henrickson, A. S. Perelson, U. H. von Andrian, and A. K. Chakraborty (2008)
Mol. Cell. Biol.
28, 4040-4051
|Abstract »|Full Text »|PDF »
{alpha}-Dystroglycan is involved in positive selection of thymocytes by participating in immunological synapse formation.
Y. Gong, R. Zhang, J. Zhang, L. Xu, F. Zhang, W. Xu, Y. Wang, Y. Chu, and S. Xiong (2008)
FASEB J
22, 1426-1439
|Abstract »|Full Text »|PDF »
CCR7 Expression in Developing Thymocytes Is Linked to the CD4 versus CD8 Lineage Decision.
X. Yin, E. Ladi, S. W. Chan, O. Li, N. Killeen, D. J. Kappes, and E. A. Robey (2007)
J. Immunol.
179, 7358-7364
|Abstract »|Full Text »|PDF »
Noninvasive Intravital Imaging of Thymocyte Dynamics in Medaka.
J. Li, N. Iwanami, V. Q. Hoa, M. Furutani-Seiki, and Y. Takahama (2007)
J. Immunol.
179, 1605-1615
|Abstract »|Full Text »|PDF »
Lymph node topology dictates T cell migration behavior.
J. B. Beltman, A. F.M. Maree, J. N. Lynch, M. J. Miller, and R. J. de Boer (2007)
J. Exp. Med.
204, 771-780
|Abstract »|Full Text »|PDF »
The quantitative assessment of MHC II on thymic epithelium: implications in cortical thymocyte development.
S. J. Yang, S. Ahn, C. S. Park, K. L Holmes, J. Westrup, C. H. Chang, and M. G Kim (2006)
Int. Immunol.
18, 729-739
|Abstract »|Full Text »|PDF »
Plasticity in the positive selection of T cells: affinity of the selecting antigen and IL-7 affect T cell responsiveness.
T Cells in G1 Provide a Memory-Like Response to Secondary Stimulation.
I. Munitic, P. E. Ryan, and J. D. Ashwell (2005)
J. Immunol.
174, 4010-4018
|Abstract »|Full Text »|PDF »
Movies, measurement, and modeling: the three Ms of mechanistic immunology.
C. Witt, S. Raychaudhuri, and A. K. Chakraborty (2005)
J. Exp. Med.
201, 501-504
|Abstract »|Full Text »|PDF »
A spectrum of biophysical interaction modes between T cells and different antigen-presenting cells during priming in 3-D collagen and in vivo.
M. Gunzer, C. Weishaupt, A. Hillmer, Y. Basoglu, P. Friedl, K. E. Dittmar, W. Kolanus, G. Varga, and S. Grabbe (2004)
Blood
104, 2801-2809
|Abstract »|Full Text »|PDF »
Direct Impact of T Cells on Neurons Revealed by Two-Photon Microscopy in Living Brain Tissue.
R. Nitsch, E. E. Pohl, A. Smorodchenko, C. Infante-Duarte, O. Aktas, and F. Zipp (2004)
J. Neurosci.
24, 2458-2464
|Abstract »|Full Text »|PDF »
Thymocyte Sensitivity and Supramolecular Activation Cluster Formation Are Developmentally Regulated: A Partial Role for Sialylation.
T. K. Starr, M. A. Daniels, M. M. Lucido, S. C. Jameson, and K. A. Hogquist (2003)
J. Immunol.
171, 4512-4520
|Abstract »|Full Text »|PDF »
Shrinking the Biologic World--Nanobiotechnologies for Toxicology.
T. J. Zieziulewicz, D. W. Unfricht, N. Hadjout, M. A. Lynes, and D. A. Lawrence (2003)
Toxicol. Sci.
74, 235-244
|Abstract »|Full Text »|PDF »
Low T cell receptor expression and thermal fluctuations contribute to formation of dynamic multifocal synapses in thymocytes.
