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Science 17 May 2002:
Vol. 296. no. 5571, pp. 1319 - 1321
DOI: 10.1126/science.1070948
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Reports

Induction and Suppression of RNA Silencing by an Animal Virus

Hongwei Li, Wan Xiang Li, Shou Wei Ding*

RNA silencing is a sequence-specific RNA degradation mechanism that is operational in plants and animals. Here, we show that flock house virus (FHV) is both an initiator and a target of RNA silencing in Drosophila host cells and that FHV infection requires suppression of RNA silencing by an FHV-encoded protein, B2. These findings establish RNA silencing as an adaptive antiviral defense in animal cells. B2 also inhibits RNA silencing in transgenic plants, providing evidence for a conserved RNA silencing pathway in the plant and animal kingdoms.

Department of Plant Pathology and Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA.
*   To whom correspondence should be addressed. E-mail: shou-wei.ding{at}ucr.edu

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Z. Wang, L. Ren, X. Zhao, T. Hung, A. Meng, J. Wang, and Y.-G. Chen (2004)
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E. V. Ryabov, R. van Wezel, J. Walsh, and Y. Hong (2004)
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Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis.
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L. Gitlin and R. Andino (2003)
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J. Virol. 77, 4481-4488
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Double-stranded RNA-binding proteins could suppress RNA interference-mediated antiviral defences.
Z. Lichner, D. Silhavy, and J. Burgyan (2003)
J. Gen. Virol. 84, 975-980
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siRNAs: a new wave of RNA-based therapeutics.
G. A. Coburn and B. R. Cullen (2003)
J. Antimicrob. Chemother. 51, 753-756
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G. Randall, A. Grakoui, and C. M. Rice (2003)
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Negative-Strand Tospoviruses and Tenuiviruses Carry a Gene for a Suppressor of Gene Silencing at Analogous Genomic Positions.
E. Bucher, T. Sijen, P. de Haan, R. Goldbach, and M. Prins (2002)
J. Virol. 77, 1329-1336
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F. Qu, T. Ren, and T. J. Morris (2002)
J. Virol. 77, 511-522
   Abstract »    Full Text »    PDF »
RNA Silencing of Dengue Virus Type 2 Replication in Transformed C6/36 Mosquito Cells Transcribing an Inverted-Repeat RNA Derived from the Virus Genome.
Z. N. Adelman, I. Sanchez-Vargas, E. A. Travanty, J. O. Carlson, B. J. Beaty, C. D. Blair, and K. E. Olson (2002)
J. Virol. 76, 12925-12933
   Abstract »    Full Text »    PDF »
Replicating hepatitis delta virus RNA is edited in the nucleus by the small form of ADAR1.
S. K. Wong and D. W. Lazinski (2002)
PNAS 99, 15118-15123
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From the Cover: A viral suppressor of RNA silencing differentially regulates the accumulation of short interfering RNAs and micro-RNAs in tobacco.
A. C. Mallory, B. J. Reinhart, D. Bartel, V. B. Vance, and L. H. Bowman (2002)
PNAS 99, 15228-15233
   Abstract »    Full Text »    PDF »
Small Interfering RNAs: A Revolutionary Tool for the Analysis of Gene Function and Gene Therapy.
T. Tuschl and A. Borkhardt (2002)
Mol. Interv. 2, 158-167
   Abstract »    Full Text »    PDF »
RNA Silencing: The Genome's Immune System.
R. H. A. Plasterk (2002)
Science 296, 1263-1265
   Abstract »    Full Text »    PDF »


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