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Science 10 May 2002:
Vol. 296. no. 5570, pp. 1124 - 1126
DOI: 10.1126/science.1070536
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Reports

Role of Delayed Nuclear Envelope Breakdown and Mitosis in Wolbachia-Induced Cytoplasmic Incompatibility

Uyen Tram, William Sullivan*

The bacterium Wolbachia manipulates reproduction in millions of insects worldwide; the most common effect is cytoplasmic incompatibility (CI). We found that CI resulted from delayed nuclear envelope breakdown of the male pronucleus in Nasonia vitripennis. This caused asynchrony between the male and female pronuclei and, ultimately, loss of paternal chromosomes at the first mitosis. When Wolbachia were present in the egg, synchrony was restored, which explains suppression of CI in these crosses. These results suggest that Wolbachia target cell cycle regulatory proteins. A striking consequence of CI is that it alters the normal pattern of reciprocal centrosome inheritance in Nasonia.

Department of Molecular, Cell, and Developmental Biology, 319 Sinsheimer Labs, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
*   To whom correspondence should be addressed. E-mail: sullivan{at}biology.ucsc.edu

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Paternal chromosome segregation during the first mitotic division determines Wolbachia-induced cytoplasmic incompatibility phenotype.
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T. Ruang-areerate and P. Kittayapong (2006)
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Interspecific transfer of Wolbachia into the mosquito disease vector Aedes albopictus.
Z. Xi, C. C.H Khoo, and S. L Dobson (2006)
Proc R Soc B 273, 1317-1322
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A Genetic Test of the Role of the Maternal Pronucleus in Wolbachia-Induced Cytoplasmic Incompatibility in Drosophila melanogaster.
P. M. Ferree and W. Sullivan (2006)
Genetics 173, 839-847
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Wolbachia Establishment and Invasion in an Aedes aegypti Laboratory Population.
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I. Iturbe-Ormaetxe, G. R. Burke, M. Riegler, and S. L. O'Neill (2005)
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S. Zabalou, M. Riegler, M. Theodorakopoulou, C. Stauffer, C. Savakis, and K. Bourtzis (2004)
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Host Genotype Determines Cytoplasmic Incompatibility Type in the Haplodiploid Genus Nasonia.
S. R. Bordenstein, J. J. Uy, and J. H. Werren (2003)
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