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Science 10 May 2002:
Vol. 296. no. 5570, pp. 1115 - 1118
DOI: 10.1126/science.1070594
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Reports

Female Germ Cell Aneuploidy and Embryo Death in Mice Lacking the Meiosis-Specific Protein SCP3

Li Yuan, Jian-Guo Liu, Mary-Rose Hoja, Johannes Wilbertz, Katarina Nordqvist,* Christer Höögdagger

Aneuploidy (trisomy or monosomy) is the leading genetic cause of pregnancy loss in humans and results from errors in meiotic chromosome segregation. Here, we show that the absence of synaptonemal complex protein 3 (SCP3) promotes aneuploidy in murine oocytes by inducing defective meiotic chromosome segregation. The abnormal oocyte karyotype is inherited by embryos, which die in utero at an early stage of development. In addition, embryo death in SCP3-deficient females increases with advancing maternal age. We found that SCP3 is required for chiasmata formation and for the structural integrity of meiotic chromosomes, suggesting that altered chromosomal structure triggers nondisjunction. SCP3 is thus linked to inherited aneuploidy in female germ cells and provides a model system for studying age-dependent degeneration in oocytes.

Center for Genomics and Bioinformatics and Department of Cell and Molecular Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
*   Present address: Molecular Sciences, Astra Zeneca R&D, SE-151 85 Södertälje, Sweden.

dagger    To whom correspondence should be addressed. E-mail: christer.hoog{at}cmb.ki.se

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