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Originally published in Science Express on 4 April 2002
Science 26 April 2002:
Vol. 296. no. 5568, pp. 755 - 758
DOI: 10.1126/science.1068943
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Reports

Transcription Control by E1A and MAP Kinase Pathway via Sur2 Mediator Subunit

Jennitte L. Stevens,1 Greg T. Cantin,1 Gang Wang,1 Andrej Shevchenko,2 Anna Shevchenko,2 Arnold J. Berk13*

Sur2 is a metazoan Mediator subunit that interacts with the adenovirus E1A protein and functions in a mitogen-activated protein kinase pathway required for vulva development in Caenorhabditis elegans. We generated sur2-/- embryonic stem cells to analyze its function as a mammalian Mediator component. Our results show that Sur2 forms a subcomplex of the Mediator with two other subunits, TRAP/Med100 and 95. Knock-out of Sur2 prevents activation by E1A-CR3 and the mitogen-activated protein kinase-regulated ETS transcription factor Elk-1, but not by multiple other transcription factors. These results imply that specific activation domains stimulate transcription by binding to distinct Mediator subunits. Activation by E1A and Elk-1 requires recruitment of Mediator to a promoter by binding to its Sur2 subunit.

1 Molecular Biology Institute, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA.
2 Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
3 Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
*   To whom correspondence should be addressed. E-mail: berk{at}mbi.ucla.edu

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