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Science 26 April 2002:
Vol. 296. no. 5568, pp. 744 - 747
DOI: 10.1126/science.1069288
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Reports

A Tomato Cysteine Protease Required for Cf-2-Dependent Disease Resistance and Suppression of Autonecrosis

Julia Krüger,1 Colwyn M. Thomas,12 Catherine Golstein,13 Mark S. Dixon,14 Matthew Smoker,1 Saijun Tang,15 Lonneke Mulder,1 Jonathan D. G. Jones1*

Little is known of how plant disease resistance (R) proteins recognize pathogens and activate plant defenses. Rcr3 is specifically required for the function of Cf-2, a Lycopersicon pimpinellifolium gene bred into cultivated tomato (Lycopersicon esculentum) for resistance to Cladosporium fulvum. Rcr3 encodes a secreted papain-like cysteine endoprotease. Genetic analysis shows Rcr3 is allelic to the L. pimpinellifolium Ne gene, which suppresses the Cf-2-dependent autonecrosis conditioned by its L. esculentum allele, ne (necrosis). Rcr3 alleles from these two species encode proteins that differ by only seven amino acids. Possible roles of Rcr3 in Cf-2-dependent defense and autonecrosis are discussed.

1 The Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK.
2 School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
3 Department of Biology, Indiana University, Bloomington, IN 47405, USA.
4 School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK.
5 Department of Biology, 108 Coker Hall, University of North Carolina, Chapel Hill, NC 27599-3280, USA.
*   To whom correspondence should be addressed. E-mail jonathan.jones{at}bbsrc.ac.uk

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