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Science 19 April 2002:
Vol. 296. no. 5567, pp. 541 - 545
DOI: 10.1126/science.1068206
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Reports

Optical Projection Tomography as a Tool for 3D Microscopy and Gene Expression Studies

James Sharpe,* Ulf Ahlgren, Paul Perry, Bill Hill, Allyson Ross, Jacob Hecksher-Sørensen, Richard Baldock, Duncan Davidson

Current techniques for three-dimensional (3D) optical microscopy (deconvolution, confocal microscopy, and optical coherence tomography) generate 3D data by "optically sectioning" the specimen. This places severe constraints on the maximum thickness of a specimen that can be imaged. We have developed a microscopy technique that uses optical projection tomography (OPT) to produce high-resolution 3D images of both fluorescent and nonfluorescent biological specimens with a thickness of up to 15 millimeters. OPT microscopy allows the rapid mapping of the tissue distribution of RNA and protein expression in intact embryos or organ systems and can therefore be instrumental in studies of developmental biology or gene function.

Medical Research Council, Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.
*   To whom correspondence should be addressed. E-mail: james.sharpe{at}hgu.mrc.ac.uk

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