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Science 29 March 2002:
Vol. 295. no. 5564, pp. 2442 - 2446
DOI: 10.1126/science.1069989
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Reports

Role of a Ubiquitin-Like Modification in Polarized Morphogenesis

Gunnar A. G. Dittmar,1 Caroline R. M. Wilkinson,1 Paul T. Jedrzejewski,2* Daniel Finley1dagger

Type I ubiquitin-like proteins constitute a family of protein modifiers. Here we report the identification of a posttranslational protein modifier from Saccharomyces cerevisiae, Hub1. Overexpression of Hub1 resulted in enhanced conjugate formation when its carboxyl-terminal residue was deleted, suggesting that mature Hub1 may be produced by proteolytic processing. In vivo targets of Hub1 conjugation included cell polarity factors Sph1 and Hbt1. In the hub1Delta mutant, the subcellular localization of both Hbt1 and Sph1 was disrupted, and cell polarization during the formation of mating projections was defective. Consistent with these polarization defects, the hub1Delta mutant was deficient in mating.

1 Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
2 Barnett Institute, Northeastern University, Boston, MA 02115, USA.
*   Present address: Genencor International, 925 Page Mill Road, Palo Alto, CA 94304, USA.

dagger    To whom correspondence should be addressed. E-mail: daniel_finley{at}hms.harvard.edu

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