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Science 8 March 2002:
Vol. 295. no. 5561, pp. 1910 - 1912
DOI: 10.1126/science.1065028
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Reports
Parallel Single-Cell Monitoring of Receptor-Triggered Membrane Translocation of a Calcium-Sensing Protein Module
Mary N. Teruel,
Tobias Meyer*
Time courses of translocation of fluorescently conjugated proteins
to the plasma membrane were simultaneously measured in thousands of
individual rat basophilic leukemia cells. We found that the C2
domain--a calcium-sensing, lipid-binding protein module that is an
essential regulator of protein kinase C and numerous other
proteins--targeted proteins to the plasma membrane transiently if
calcium was released from internal stores, and persistently in response
to entry of extracellular calcium across the plasma membrane. The C2
domain translocation time courses of stimulated cells clustered into
only two primary modes. Hence, the reversible recruitment of families
of signaling proteins from one cellular compartment to another is a
rapid bifurcation mechanism for inducing discrete states of cellular
signaling networks.
Department of Molecular Pharmacology, Stanford University Medical
School, 269 Campus Drive, Stanford, CA 94305, USA.
*
To whom correspondence should be addressed. E-mail:
tobiasmeyer{at}stanford.edu
Read the Full Text
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