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Science 1 March 2002:
Vol. 295. no. 5560, pp. 1722 - 1726
DOI: 10.1126/science.295.5560.1722
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Reports

A Functional Screen for the Type III (Hrp) Secretome of the Plant Pathogen Pseudomonas syringae

David S. Guttman,1*dagger Boris A. Vinatzer,2*dagger Sara F. Sarkar,1 Max V. Ranall,2 Gregory Kettler,2 Jean T. Greenberg2

Type III secreted "effector" proteins of bacterial pathogens play central roles in virulence, yet are notoriously difficult to identify. We used an in vivo genetic screen to identify 13 effectors secreted by the type III apparatus (called Hrp, for "hypersensitive response and pathogenicity") of the plant pathogen Pseudomonas syringae. Although sharing little overall homology, the amino-terminal regions of these effectors had strikingly similar amino acid compositions. This feature facilitated the bioinformatic prediction of 38 P. syringae effectors, including 15 previously unknown proteins. The secretion of two of these putative effectors was shown to be type III-dependent. Effectors showed high interstrain variation, supporting a role for some effectors in adaptation to different hosts.

1 Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, ON M5S 3B2, Canada.
2 Department of Molecular Genetics and Cell Biology, University of Chicago, 1103 East 57th Street, Chicago, IL 60637, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: guttman{at}botany.utoronto.ca

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Science. ISSN 0036-8075 (print), 1095-9203 (online)