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Originally published in Science Express on 7 February 2002
Science 1 March 2002:
Vol. 295. no. 5560, pp. 1702 - 1705
DOI: 10.1126/science.1067172
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Reports

Protein Nanoarrays Generated By Dip-Pen Nanolithography

Ki-Bum Lee,1 So-Jung Park,1 Chad A. Mirkin,1* Jennifer C. Smith,2 Milan Mrksich2*

Dip-pen nanolithography was used to construct arrays of proteins with 100- to 350-nanometer features. These nanoarrays exhibit almost no detectable nonspecific binding of proteins to their passivated portions even in complex mixtures of proteins, and therefore provide the opportunity to study a variety of surface-mediated biological recognition processes. For example, reactions involving the protein features and antigens in complex solutions can be screened easily by atomic force microscopy. As further proof-of-concept, these arrays were used to study cellular adhesion at the submicrometer scale.

1 Northwestern University, Department of Chemistry and Center for Nanofabrication and Molecular Self-Assembly, 2145 Sheridan Road, Evanston, IL 60208, USA.
2 University of Chicago, Department of Chemistry and the Institute for Biophysical Dynamics, 5735 South Ellis, Chicago, IL 60637, USA.
*   To whom correspondence should be addressed. E-mail: camirkin{at}chem.northwestern.edu; for cell adhesion work: mmrksich{at}midway.uchicago.edu

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