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Originally published in Science Express on 17 January 2002
Science 22 February 2002:
Vol. 295. no. 5559, pp. 1536 - 1539
DOI: 10.1126/science.1064913
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Reports

Retrograde Support of Neuronal Survival Without Retrograde Transport of Nerve Growth Factor

Bronwyn L. MacInnis, Robert B. Campenot*

Application of nerve growth factor (NGF) covalently cross-linked to beads increased the phosphorylation of TrkA and Akt, but not of mitogen-activated protein kinase, in cultured rat sympathetic neurons. NGF beads or iodine-125-labeled NGF beads supplied to distal axons resulted in the survival of over 80% of the neurons for 30 hours, with little or no retrograde transport of iodine-125-labeled NGF; whereas application of free iodine-125-labeled NGF (0.5 nanograms per milliliter) produced 20-fold more retrograde transport, but only 29% of the neurons survived. Thus, in contrast to widely accepted theory, a neuronal survival signal can reach the cell bodies unaccompanied by the NGF that initiated it.

Department of Cell Biology, 6-14 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada, T6G 2H7.
*   To whom correspondence should be addressed. E-mail: bob.campenot{at}ualberta.ca

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