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Science 15 February 2002:
Vol. 295. no. 5558, pp. 1299 - 1301
DOI: 10.1126/science.1065769
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Reports

Regulation of Brassinosteroid Signaling by a GSK3/SHAGGY-Like Kinase

Jianming Li,* Kyoung Hee Nam

GSK3/SHAGGY is a highly conserved serine/threonine kinase implicated in many signaling pathways in eukaryotes. Although many GSK3/SHAGGY-like kinases have been identified in plants, little is known about their functions in plant growth and development. Here we show that the Arabidopsis BRASSINOSTEROID-INSENSITIVE 2 (BIN2) gene encodes a GSK3/SHAGGY-like kinase. Gain-of-function mutations within its coding sequence or its overexpression inhibit brassinosteroid (BR) signaling, resulting in plants that resemble BR-deficient and BR-response mutants. In contrast, reduced BIN2 expression via cosuppression partially rescues a weak BR-signaling mutation. Thus, BIN2 acts as a negative regulator to control steroid signaling in plants.

Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, USA.
*   To whom correspondence should be addressed. E-mail: jian{at}umich.edu

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