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Science 15 February 2002:
Vol. 295. no. 5558, pp. 1294 - 1298
DOI: 10.1126/science.1067765
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Reports

Cytoskeletal Regulation by the Nedd8 Ubiquitin-Like Protein Modification Pathway

Thimo Kurz,1 Lionel Pintard,2 John H. Willis,1 Danielle R. Hamill,1* Pierre Gönczy,2 Matthias Peter,2 Bruce Bowerman1dagger

The Nedd8 ubiquitin-like protein modification pathway regulates cell-cycle progression. Our analysis of Nedd8 requirements during Caenorhabditis elegans embryogenesis indicates that the cytoskeleton is another target. Nedd8 conjugation negatively regulated contractility of the microfilament-rich cell cortex during pronuclear migration and again during cytokinesis. The Nedd8 pathway also was required after meiosis to negatively regulate katanin, a microtubule-severing complex, permitting the assembly of a large mitotic spindle. We propose that Nedd8-modified cullin, as part of an E3 ubiquitin ligase complex, targets katanin for degradation during the transition from meiosis to mitosis.

1 Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
2 Swiss Institute for Experimental Cancer Research (ISREC), CH-1066 Lausanne, Switzerland.
*   Present address: Department of Zoology, Ohio Wesleyan University, Delaware, OH 43015, USA.

dagger    To whom correspondence should be addressed. E-mail: bbowerman{at}molbio.uoregon.edu

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