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Originally published in Science Express on 10 January 2002
Science 1 February 2002:
Vol. 295. no. 5556, pp. 868 - 872
DOI: 10.1126/science.1067081
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Reports

Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors

Carlos Lois,* Elizabeth J. Hong,* Shirley Pease, Eric J. Brown, David Baltimoredagger

Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the transgene(s) and displayed green fluorescence. Mice generated using lentiviral vectors with muscle-specific and T lymphocyte-specific promoters expressed high levels of GFP only in the appropriate cell types. We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species.

Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: baltimo{at}caltech.edu

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P. Maurel, S. Einheber, J. Galinska, P. Thaker, I. Lam, M. B. Rubin, S. S. Scherer, Y. Murakami, D. H. Gutmann, and J. L. Salzer (2007)
J. Cell Biol. 178, 861-874
   Abstract »    Full Text »    PDF »
Regulation of Neurite Growth by Spontaneous Ca2+ Oscillations in Astrocytes.
K. Kanemaru, Y. Okubo, K. Hirose, and M. Iino (2007)
J. Neurosci. 27, 8957-8966
   Abstract »    Full Text »    PDF »
Role for DNA repair factor XRCC4 in immunoglobulin class switch recombination.
P. Soulas-Sprauel, G. Le Guyader, P. Rivera-Munoz, V. Abramowski, C. Olivier-Martin, C. Goujet-Zalc, P. Charneau, and J.-P. de Villartay (2007)
J. Exp. Med. 204, 1717-1727
   Abstract »    Full Text »    PDF »
Efficient transgenic rat production by a lentiviral vector.
M. Michalkiewicz, T. Michalkiewicz, A. M. Geurts, R. J. Roman, G. R. Slocum, O. Singer, D. Weihrauch, A. S. Greene, M. Kaldunski, I. M. Verma, et al. (2007)
Am J Physiol Heart Circ Physiol 293, H881-H894
   Abstract »    Full Text »    PDF »
Nodes of Ranvier and axon initial segments are ankyrin G-dependent domains that assemble by distinct mechanisms.
Y. Dzhashiashvili, Y. Zhang, J. Galinska, I. Lam, M. Grumet, and J. L. Salzer (2007)
J. Cell Biol. 177, 857-870
   Abstract »    Full Text »    PDF »
Conditional ablation of GFR{alpha}1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung's disease phenotype.
T. Uesaka, S. Jain, S. Yonemura, Y. Uchiyama, J. Milbrandt, and H. Enomoto (2007)
Development 134, 2171-2181
   Abstract »    Full Text »    PDF »
Soluble Ig-Like Transcript 3 Inhibits Tumor Allograft Rejection in Humanized SCID Mice and T Cell Responses in Cancer Patients.
N. Suciu-Foca, N. Feirt, Q.-Y. Zhang, G. Vlad, Z. Liu, H. Lin, C.-C. Chang, E. K. Ho, A. I. Colovai, H. Kaufman, et al. (2007)
J. Immunol. 178, 7432-7441
   Abstract »    Full Text »    PDF »
Control of Early Events in Olfactory Processing by Adult Neurogenesis.
G. Gheusi and P.-M. Lledo (2007)
Chem Senses 32, 397-409
   Abstract »    Full Text »    PDF »
Direct Intramyocardial But Not Intracoronary Injection of Bone Marrow Cells Induces Ventricular Arrhythmias in a Rat Chronic Ischemic Heart Failure Model.
S. Fukushima, A. Varela-Carver, S. R. Coppen, K. Yamahara, L. E. Felkin, J. Lee, P. J.R. Barton, C. M.N. Terracciano, M. H. Yacoub, and K. Suzuki (2007)
Circulation 115, 2254-2261
   Abstract »    Full Text »    PDF »
Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors: Zona Pellucida Removal and Subzonal Injection Methods.
O. Singer, G. Tiscornia, and I. M. Verma (2007)
CSH Protocols 2007, pdb.prot4756
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Dorsal Radial Glia Generate Olfactory Bulb Interneurons in the Postnatal Murine Brain.
R. E. Ventura and J. E. Goldman (2007)
J. Neurosci. 27, 4297-4302
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NFX1-123 and Poly(A) Binding Proteins Synergistically Augment Activation of Telomerase in Human Papillomavirus Type 16 E6-Expressing Cells.
R. A. Katzenellenbogen, E. M. Egelkrout, P. Vliet-Gregg, L. C. Gewin, P. R. Gafken, and D. A. Galloway (2007)
J. Virol. 81, 3786-3796
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Sulfated Homologues of Heparin Inhibit Hepatitis C Virus Entry into Mammalian Cells.
A. Basu, T. Kanda, A. Beyene, K. Saito, K. Meyer, and R. Ray (2007)
J. Virol. 81, 3933-3941
   Abstract »    Full Text »    PDF »
Targeted Transgene Expression in Muller Glia of Normal and Diseased Retinas Using Lentiviral Vectors.
K. P. Greenberg, S. F. Geller, D. V. Schaffer, and J. G. Flannery (2007)
Invest. Ophthalmol. Vis. Sci. 48, 1844-1852
   Abstract »    Full Text »    PDF »
Enhanced Glucocorticoid Receptor Signaling in T Cells Impacts Thymocyte Apoptosis and Adaptive Immune Responses.
J. v. d. Brandt, F. Luhder, K. G. McPherson, K. L. de Graaf, D. Tischner, S. Wiehr, T. Herrmann, R. Weissert, R. Gold, and H. M. Reichardt (2007)
Am. J. Pathol. 170, 1041-1053
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Inducible and Reversible Transgene Expression in Human Stem Cells After Efficient and Stable Gene Transfer.
B. Y. Zhou, Z. Ye, G. Chen, Z. P. Gao, Y. A. Zhang, and L. Cheng (2007)
Stem Cells 25, 779-789
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Synapse-Associated Protein-97 Mediates {alpha}-Secretase ADAM10 Trafficking and Promotes Its Activity.
E. Marcello, F. Gardoni, D. Mauceri, S. Romorini, A. Jeromin, R. Epis, B. Borroni, F. Cattabeni, C. Sala, A. Padovani, et al. (2007)
J. Neurosci. 27, 1682-1691
   Abstract »    Full Text »    PDF »
From the Cover: Oviduct-specific expression of two therapeutic proteins in transgenic hens.
S. G. Lillico, A. Sherman, M. J. McGrew, C. D. Robertson, J. Smith, C. Haslam, P. Barnard, P. A. Radcliffe, K. A. Mitrophanous, E. A. Elliot, et al. (2007)
PNAS 104, 1771-1776
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