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Science 1 February 2002:
Vol. 295. no. 5556, pp. 855 - 857
DOI: 10.1126/science.1066303
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Reports
A Role for Interaction of the RNA Polymerase Flap Domain with the  Subunit in Promoter Recognition
Konstantin Kuznedelov,1*
Leonid Minakhin,1*
Anita Niedziela-Majka,2
Simon L. Dove,3
Dragana Rogulja,1
Bryce E. Nickels,3
Ann Hochschild,3
Tomasz Heyduk,2
Konstantin Severinov1§
In bacteria, promoter recognition depends on the RNA
polymerase  subunit, which combines with the catalytically
proficient RNA polymerase core to form the holoenzyme. The major class
of bacterial promoters is defined by two conserved elements (the -10
and -35 elements, which are 10 and 35 nucleotides upstream of the
initiation point, respectively) that are contacted by in the
holoenzyme. We show that recognition of promoters of this class depends
on the "flexible flap" domain of the RNA polymerase  subunit.
The flap interacts with conserved region 4 of and triggers a
conformational change that moves region 4 into the correct position for
interaction with the -35 element. Because the flexible flap is
evolutionarily conserved, this domain may facilitate promoter
recognition by specificity factors in eukaryotes as well.
1 Waksman Institute, Department of Genetics,
Rutgers University, Piscataway, NJ 08854, USA.
2 E. A. Doisy Department of Biochemistry and
Molecular Biology, St. Louis University Medical School, St. Louis, MO
63104, USA.
3 Harvard Medical School, Department of
Microbiology and Molecular Genetics, Boston, MA 02115, USA.
*
These authors contributed equally to this work.
On leave from Limnological Institute of the Russian
Academy of Sciences, Irkutsk, Russia.
On leave from Department of Organic Chemistry,
Biochemistry and Biotechnology, Wroclaw University of Technology,
Wroclaw, Poland.
§
To whom correspondence should be addressed. E-mail:
severik{at}waksman.rutgers.edu
Read the Full Text
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