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Science 1 February 2002:
Vol. 295. no. 5556, pp. 848 - 851
DOI: 10.1126/science.1068064
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Reports

Partitioning of the Matrix Fraction of the Golgi Apparatus During Mitosis in Animal Cells

Joachim Seemann, Marc Pypaert, Tomohiko Taguchi, Jörg Malsam, Graham Warren*

The Golgi apparatus is partitioned during mitosis in animal cells by a process of fragmentation, dispersal, and reassembly in each daughter cell. We fractionated the Golgi apparatus in vivo using the drug brefeldin A or a dominant-negative mutant of the Sar1p protein. After these treatments, Golgi enzymes moved back to the endoplasmic reticulum, leaving behind a matrix of Golgi structural proteins. Under these conditions, cells still entered and exited mitosis normally, and their Golgi matrix partitioned in a manner very similar to that of the complete organelle. Thus, the matrix may be the partitioning unit of the Golgi apparatus and may carry the Golgi enzyme-containing membranes into the daughter cells.

Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520-8002, USA.
*   To whom correspondence should be addressed. E-mail: graham.warren{at}yale.edu

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