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Science 25 January 2002:
Vol. 295. no. 5555, pp. 669 - 671
DOI: 10.1126/science.1066648
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Reports

Role of the Myosin Assembly Protein UNC-45 as a Molecular Chaperone for Myosin

José M. Barral,1*dagger Alex H. Hutagalung,1* Achim Brinker,3 F. Ulrich Hartl,3 Henry F. Epstein12ddagger

The organization of myosin into motile cellular structures requires precise temporal and spatial regulation. Proteins containing a UCS (UNC-45/CRO1/She4p) domain are necessary for the incorporation of myosin into the contractile ring during cytokinesis and into thick filaments during muscle development. We report that the carboxyl-terminal regions of UNC-45 bound and exerted chaperone activity on the myosin head. The amino-terminal tetratricopeptide repeat domain of UNC-45 bound the molecular chaperone Hsp90. Thus, UNC-45 functions both as a molecular chaperone and as an Hsp90 co-chaperone for myosin, which can explain previous findings of altered assembly and decreased accumulation of myosin in UNC-45 mutants of Caenorhabditis elegans.

1 Department of Biochemistry and Molecular Biology,
2 Department of Neurology, Baylor College of Medicine, Houston, TX 77030, USA.
3 Max Planck Institute for Biochemistry, D82152 Martinsried, Germany.
*   These authors contributed equally to this work.

dagger    Present address: Max Planck Institute for Biochemistry, D82152 Martinsried, Germany.

ddagger    To whom correspondence should be addressed. E-mail: hepstein{at}bcm.tmc.edu

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