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Science 4 January 2002:
Vol. 295. no. 5552, pp. 147 - 150
DOI: 10.1126/science.1064281
Prev | Table of Contents

Reports

Sitosterol-beta -glucoside as Primer for Cellulose Synthesis in Plants

Liangcai Peng,* Yasushi Kawagoe,dagger Pat Hogan, Deborah Delmerddagger

Cellulose synthesis in plants requires beta -1,4-glucan chain initiation, elongation, and termination. The process of chain elongation is likely to be distinct from the process of chain initiation. We demonstrate that a CesA glucosyltransferase initiates glucan polymerization by using sitosterol-beta -glucoside (SG) as primer. Cotton fiber membranes synthesize sitosterol-cellodextrins (SCDs) from SG and uridine 5'-diphosphate-glucose (UDP-Glc) under conditions that also favor cellulose synthesis. The cellulase encoded by the Korrigan (Kor) gene, required for cellulose synthesis in plants, may function to cleave SG from the growing polymer chain.

Section of Plant Biology, University of California Davis, One Shields Avenue, Davis, CA 95616, USA.
*   Present address: Plant Gene Expression Center, 800 Buchanan Street, Albany CA 94710-1198, USA.

dagger    Present address: National Institute of Agrobiological Resources, Kannondai 2-1-2, Tsukuba, Ibaraki, Japan 305-8602.

ddagger    To whom correspondence should be addressed: E-mail: dpdelmer{at}ucdavis.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)