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Science 4 January 2002:
Vol. 295. no. 5552, pp. 131 - 134
DOI: 10.1126/science.1065199
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Reports

Construction and Analysis of a Human-Chimpanzee Comparative Clone Map

Asao Fujiyama,12*dagger Hidemi Watanabe,1*dagger Atsushi Toyoda,1* Todd D. Taylor,1* Takehiko Itoh,3* Shih-Feng Tsai,45* Hong-Seog Park,6* Marie-Laure Yaspo,7* Hans Lehrach,7 Zhu Chen,8* Gang Fu,8* Naruya Saitou,2* Kazutoyo Osoegawa,9 Pieter J. de Jong,9 Yumiko Suto,10 Masahira Hattori,1*dagger Yoshiyuki Sakaki111*dagger

The recently released human genome sequences provide us with reference data to conduct comparative genomic research on primates, which will be important to understand what genetic information makes us human. Here we present a first-generation human-chimpanzee comparative genome map and its initial analysis. The map was constructed through paired alignment of 77,461 chimpanzee bacterial artificial chromosome end sequences with publicly available human genome sequences. We detected candidate positions, including two clusters on human chromosome 21 that suggest large, nonrandom regions of difference between the two genomes.

1 RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
2 National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8450, Japan.
3 Mitsubishi Research Institute, 3-6, Otemachi 2-chome Chiyoda-ku, Tokyo 100-8141, Japan.
4 National Yang-Ming University, 128, Yen-chiu, Yuan Road, Sec. 2, Taipei 115, Taiwan.
5 National Health Research Institutes, 128, Yen-chiu, Yuan Road, Sec. 2, Taipei 115, Taiwan.
6 Genome Research Center, KRIBB, 52 Oun-dong, Yusong-gu, Daejeon 305-333, Korea.
7 Max Planck Institute for Molecular Genetics, Ihnestrasse 73, D-14195 Berlin-Dahlem, Germany.
8 Chinese National Human Genome Center at Shanghai, 351, Guo Shou Jing Road, Shanghai 201203, China.
9 Children's Hospital Oakland Research Institute, BACPAC Resources, 747 52nd Street, Oakland, CA 94609-1809, USA.
10 Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongou, Bunkyou-ku, Tokyo 113-0033, Japan.
11 Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
*   These authors form the International Consortium for the Sequencing of Chimpanzee Chromosome 22.

dagger    To whom correspondence should be addressed. E-mail: afujiyam{at}gsc.riken.go.jp, watanabe{at}gsc.riken.go.jp, hattori{at}gsc.riken.go.jp, sakaki{at}gsc.riken.go.jp

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