Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 4 January 2002:
Vol. 295. no. 5552, pp. 124 - 127
DOI: 10.1126/science.1065570
Prev | Table of Contents | Next

Reports

Germ Cell Survival Through Carbohydrate-Mediated Interaction with Sertoli Cells

Tomoya O. Akama,1 Hiroaki Nakagawa,13 Kazuhiro Sugihara,14 Sonoko Narisawa,2 Chikara Ohyama,5 Shin-Ichiro Nishimura,3 Deborah A. O'Brien,6 Kelley W. Moremen,7 José Luis Millán,2 Michiko N. Fukuda1*

Spermatogenesis is a precisely regulated process in which the germ cells closely interact with Sertoli cells. The molecular basis of this cell-cell adhesion is unknown. Here, we demonstrate that targeted disruption of Man2a2, a gene encoding alpha -mannosidase IIx (MX), an enzyme that forms intermediate asparagine-linked carbohydrates (N-glycans), results in Man2a2 null males that are largely infertile. The Man2a2 null spermatogenic cells fail to adhere to Sertoli cells and are prematurely released from the testis to epididymis. We identified an N-glycan structure that plays a key role in germ cell-Sertoli cell adhesion and showed that a specific carbohydrate was required for spermatogenesis.

1 Glycobiology Program and
2 Stem Cell Program, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
3 Biological Science, Graduate School of Science, Hokkaido University, Sapporo, Japan.
4 Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
5 Department of Urology, Tohoku University, Sendai, Japan.
6 Departments of Cell & Developmental Biology and Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
7 Complex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
*   To whom correspondence should be addressed. E-mail: michiko{at}burnham-inst.org

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
T Cell Receptor Signaling Co-regulates Multiple Golgi Genes to Enhance N-Glycan Branching.
H.-L. Chen, C. F. Li, A. Grigorian, W. Tian, and M. Demetriou (2009)
J. Biol. Chem. 284, 32454-32461
   Abstract »    Full Text »    PDF »
Regulation of Glycan Structures in Animal Tissues: TRANSCRIPT PROFILING OF GLYCAN-RELATED GENES.
A. V. Nairn, W. S. York, K. Harris, E. M. Hall, J. M. Pierce, and K. W. Moremen (2008)
J. Biol. Chem. 283, 17298-17313
   Abstract »    Full Text »    PDF »
Self-recognition of N-linked glycans with multivalent GlcNAc, determined as ceramide mimetic conjugate.
S.-J. Yoon, S. Ikeda, M. Sadilek, S.-i. Hakomori, H. Ishida, and M. Kiso (2007)
Glycobiology 17, 1007-1014
   Abstract »    Full Text »    PDF »
Respiratory Distress and Neonatal Lethality in Mice Lacking Golgi {alpha}1,2-Mannosidase IB Involved in N-Glycan Maturation.
L. O. Tremblay, E. N. Kovacs, E. Daniels, N. K. Wong, M. Sutton-Smith, H. R. Morris, A. Dell, E. Marcinkiewicz, N. G. Seidah, C. McKerlie, et al. (2007)
J. Biol. Chem. 282, 2558-2566
   Abstract »    Full Text »    PDF »
Unusual N-Glycan Structures in {alpha}-Mannosidase II/IIx Double Null Embryos Identified by a Systematic Glycomics Approach Based on Two-dimensional LC Mapping and Matrix-dependent Selective Fragmentation Method in MALDI-TOF/TOF Mass Spectrometry.
M. Hato, H. Nakagawa, M. Kurogochi, T. O. Akama, J. D. Marth, M. N. Fukuda, and S.-I. Nishimura (2006)
Mol. Cell. Proteomics 5, 2146-2157
   Abstract »    Full Text »    PDF »
Essential and mutually compensatory roles of {alpha}-mannosidase II and {alpha}-mannosidase IIx in N-glycan processing in vivo in mice.
T. O. Akama, H. Nakagawa, N. K. Wong, M. Sutton-Smith, A. Dell, H. R. Morris, J. Nakayama, S.-I. Nishimura, A. Pai, K. W. Moremen, et al. (2006)
PNAS 103, 8983-8988
   Abstract »    Full Text »    PDF »
Regulation of human male germ cell death by modulators of ATP production.
K. Erkkila, S. Kyttanen, M. Wikstrom, K. Taari, A. P. S. Hikim, R. S. Swerdloff, and L. Dunkel (2006)
Am J Physiol Endocrinol Metab 290, E1145-E1154
   Abstract »    Full Text »    PDF »
Experimental validation of the regulated expression of large numbers of non-coding RNAs from the mouse genome.
T. Ravasi, H. Suzuki, K. C. Pang, S. Katayama, M. Furuno, R. Okunishi, S. Fukuda, K. Ru, M. C. Frith, M. M. Gongora, et al. (2006)
Genome Res. 16, 11-19
   Abstract »    Full Text »    PDF »
Bisecting GlcNAc mediates the binding of annexin V to Hsp47.
C.-x. Gao, E. Miyoshi, N. Uozumi, R. Takamiya, X. Wang, K. Noda, J. Gu, K. Honke, Y. Wada, and N. Taniguchi (2005)
Glycobiology 15, 1067-1075
   Abstract »    Full Text »    PDF »
From The Cover: Dysregulation of TGF-{beta}1 receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient mice.
X. Wang, S. Inoue, J. Gu, E. Miyoshi, K. Noda, W. Li, Y. Mizuno-Horikawa, M. Nakano, M. Asahi, M. Takahashi, et al. (2005)
PNAS 102, 15791-15796
   Abstract »    Full Text »    PDF »
Mammalian male germ cells are fertile ground for expression profiling of sexual reproduction.
G. Wrobel and M. Primig (2005)
Reproduction 129, 1-7
   Abstract »    Full Text »    PDF »
Increased recurrence risk in congenital disorders of glycosylation type Ia (CDG-Ia) due to a transmission ratio distortion.
E Schollen, S Kjaergaard, T Martinsson, S Vuillaumier-Barrot, M Dunoe, L Keldermans, N Seta, and G Matthijs (2004)
J. Med. Genet. 41, 877-880
   Full Text »    PDF »
Mechanism-based Fluorescent Labeling of {beta}-Galactosidases: AN EFFICIENT METHOD IN PROTEOMICS FOR GLYCOSIDE HYDROLASES.
M. Kurogochi, S.-I. Nishimura, and Y. C. Lee (2004)
J. Biol. Chem. 279, 44704-44712
   Abstract »    Full Text »    PDF »
HMMC-1: A Humanized Monoclonal Antibody With Therapeutic Potential Against Mullerian Duct-Related Carcinomas.
S. Nozawa, D. Aoki, K. Tsukazaki, N. Susumu, M. Sakayori, N. Suzuki, A. Suzuki, R. Wakita, M. Mukai, Y. Egami, et al. (2004)
Clin. Cancer Res. 10, 7071-7078
   Abstract »    Full Text »    PDF »
Natural Antibiotic Function of a Human Gastric Mucin Against Helicobacter pylori Infection.
M. Kawakubo, Y. Ito, Y. Okimura, M. Kobayashi, K. Sakura, S. Kasama, M. N. Fukuda, M. Fukuda, T. Katsuyama, and J. Nakayama (2004)
Science 305, 1003-1006
   Abstract »    Full Text »    PDF »
A Novel Human {beta}1,3-N-Acetylgalactosaminyltransferase That Synthesizes a Unique Carbohydrate Structure, GalNAc{beta}1-3GlcNAc.
T. Hiruma, A. Togayachi, K. Okamura, T. Sato, N. Kikuchi, Y.-D. Kwon, A. Nakamura, K. Fujimura, M. Gotoh, K. Tachibana, et al. (2004)
J. Biol. Chem. 279, 14087-14095
   Abstract »    Full Text »    PDF »
Expression of carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) in normal human Sertoli cells and its up-regulation in impaired spermatogenesis.
H. Lauke, N. Kilic, R. Bozorgzad, M. Fernando, S. Neshat-Vahid, T. Pottek, and S. Ergun (2004)
Mol. Hum. Reprod. 10, 247-252
   Abstract »    Full Text »    PDF »
Regulated Expression and Ultrastructural Localization of Galectin-1, a Proapoptotic {beta}-Galactoside-Binding Lectin, During Spermatogenesis in Rat Testis.
L. Dettin, N. Rubinstein, A. Aoki, G. A. Rabinovich, and C. A. Maldonado (2003)
Biol Reprod 68, 51-59
   Abstract »    Full Text »    PDF »
Male Contraception.
R. A. Anderson and D. T. Baird (2002)
Endocr. Rev. 23, 735-762
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)