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Science 14 December 2001:
Vol. 294. no. 5550, pp. 2372 - 2375
DOI: 10.1126/science.1065635
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Reports

A Prokaryotic Voltage-Gated Sodium Channel

Dejian Ren,1* Betsy Navarro,12* Haoxing Xu,1* Lixia Yue,1* Qing Shi,1 David E. Clapham1dagger

The pore-forming subunits of canonical voltage-gated sodium and calcium channels are encoded by four repeated domains of six-transmembrane (6TM) segments. We expressed and characterized a bacterial ion channel (NaChBac) from Bacillus halodurans that is encoded by one 6TM segment. The sequence, especially in the pore region, is similar to that of voltage-gated calcium channels. The expressed channel was activated by voltage and was blocked by calcium channel blockers. However, the channel was selective for sodium. The identification of NaChBac as a functionally expressed bacterial voltage-sensitive ion-selective channel provides insight into both voltage-dependent activation and divalent cation selectivity.

1 Howard Hughes Medical Institute, Children's Hospital, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, MA 02115, USA.
2 Centro Internacional de Fisica, lab. de Biofisica, Bogota, Colombia.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: dclapham{at}enders.tch.harvard.edu

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