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Science 14 December 2001:
Vol. 294. no. 5550, pp. 2361 - 2364
DOI: 10.1126/science.1064603
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Reports

Visualization of DNA Replication on Individual Epstein-Barr Virus Episomes

Paolo Norio,* Carl L. Schildkraut*

The duplication of the mammalian genome is an organized event, but there is limited information about the precision of the duplication program at specific genetic loci. We developed an approach that allows DNA replication events to be visualized in individual DNA molecules. Studying the latent replication of Epstein-Barr virus episomes, we show that different initiation sites are used to commence DNA replication from a specific portion of the viral genome (zone), whereas termination does not seem to be genomically defined. We conclude that initiation zones and pausing sites are major organizers of the duplication program, but initiation, fork progression, and termination of replication can vary in each molecule.

Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
*   To whom correspondence should be addressed. E-mail: norio{at}aecom.yu.edu; schildkr{at}aecom.yu.edu

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Epstein-Barr Virus Episome Stability Is Coupled to a Delay in Replication Timing.
J. Zhou, A. R. Snyder, and P. M. Lieberman (2009)
J. Virol. 83, 2154-2162
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Linear Derivatives of Saccharomyces cerevisiae Chromosome III Can Be Maintained in the Absence of Autonomously Replicating Sequence Elements.
A. Dershowitz, M. Snyder, M. Sbia, J. H. Skurnick, L. Y. Ong, and C. S. Newlon (2007)
Mol. Cell. Biol. 27, 4652-4663
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Replication Fork Stalling at Natural Impediments.
E. V. Mirkin and S. M. Mirkin (2007)
Microbiol. Mol. Biol. Rev. 71, 13-35
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DNA Replication Origins Fire Stochastically in Fission Yeast.
P. K. Patel, B. Arcangioli, S. P. Baker, A. Bensimon, and N. Rhind (2006)
Mol. Biol. Cell 17, 308-316
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Changes in Replication, Nuclear Location, and Expression of the Igh Locus after Fusion of a Pre-B Cell Line with a T Cell Line.
J. Zhou, S. Saleque, O. Ermakova, M. A. Sepulveda, Q. Yang, L. A. Eckhardt, C. L. Schildkraut, and B. K. Birshtein (2005)
J. Immunol. 175, 2317-2320
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DNA Replication Origin Plasticity and Perturbed Fork Progression in Human Inverted Repeats.
R. Lebofsky and A. Bensimon (2005)
Mol. Cell. Biol. 25, 6789-6797
   Abstract »    Full Text »    PDF »
Telomere Repeat Binding Factors TRF1, TRF2, and hRAP1 Modulate Replication of Epstein-Barr Virus OriP.
Z. Deng, C. Atanasiu, J. S. Burg, D. Broccoli, and P. M. Lieberman (2003)
J. Virol. 77, 11992-12001
   Abstract »    Full Text »    PDF »


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