Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Applied Bio

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 7 December 2001:
Vol. 294. no. 5549, pp. 2176 - 2178
DOI: 10.1126/science.1063415
Prev | Table of Contents | Next

Reports

Facilitation of Calmodulin-Mediated Odor Adaptation by cAMP-Gated Channel Subunits

Jonathan Bradley,12* Dirk Reuter,1dagger Stephan Frings1ddagger

Calcium (Ca2+) influx through Ca2+-permeable ion channels plays a pivotal role in a variety of neuronal signaling processes, and negative-feedback control of this influx by Ca2+ itself is often equally important for modulation of such signaling. Negative modulation by Ca2+ through calmodulin (CaM) on cyclic nucleotide-gated (CNG) channels underlies the adaptation of olfactory receptor neurons to odorants. We show that this feedback requires two additional subunits of the native olfactory channel, CNGA4 and CNGB1b, even though the machinery for CaM binding and modulation is present in the principal subunit CNGA2. This provides a rationale for the presence of three distinct subunits in the native olfactory channel and underscores the subtle link between the molecular make-up of an ion channel and the physiological function it subserves.

1 Institut für Biologische Informationsverarbeitung, Forschungszentrum Jülich, 52425 Jülich, Germany.
2 Laboratoire de Neurobiologie, Ecole Normale Supérieure, 75230 Paris, France.
*   Present address: Howard Hughes Medical Institute, Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

dagger    Present address: Sophion Bioscience, Pederstrupvej 93, 2750 Ballerup, Denmark.

ddagger    To whom correspondence should be addressed. E-mail: s.frings{at}fz-juelich.de

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Electrochemical Basis of Odor Transduction in Vertebrate Olfactory Cilia.
S. J. Kleene (2008)
Chem Senses
   Abstract »    Full Text »    PDF »
Loss of CNGB1 Protein Leads to Olfactory Dysfunction and Subciliary Cyclic Nucleotide-gated Channel Trapping.
S. Michalakis, J. Reisert, H. Geiger, C. Wetzel, X. Zong, J. Bradley, M. Spehr, S. Huttl, A. Gerstner, A. Pfeifer, et al. (2006)
J. Biol. Chem. 281, 35156-35166
   Abstract »    Full Text »    PDF »
Interplay between PIP3 and calmodulin regulation of olfactory cyclic nucleotide-gated channels.
J. D. Brady, E. D. Rich, J. R. Martens, J. W. Karpen, M. D. Varnum, and R. L. Brown (2006)
PNAS 103, 15635-15640
   Abstract »    Full Text »    PDF »
Odorant Inhibition of the Olfactory Cyclic Nucleotide-gated Channel with a Native Molecular Assembly.
T.-Y. Chen, H. Takeuchi, and T. Kurahashi (2006)
J. Gen. Physiol. 128, 365-371
   Abstract »    Full Text »    PDF »
The Sweet Smell of Success: Conclusive Evidence that Cyclic AMP Hydrolysis Does Not Trigger Fast Adaptation in Olfactory Receptor Cells.
A. L. Zimmerman (2006)
J. Gen. Physiol. 128, 149-151
   Full Text »    PDF »
Fast Adaptation in Mouse Olfactory Sensory Neurons Does Not Require the Activity of Phosphodiesterase.
A. Boccaccio, L. Lagostena, V. Hagen, and A. Menini (2006)
J. Gen. Physiol. 128, 171-184
   Abstract »    Full Text »    PDF »
Model of Calcium Oscillations Due to Negative Feedback in Olfactory Cilia.
J. Reidl, P. Borowski, A. Sensse, J. Starke, M. Zapotocky, and M. Eiswirth (2006)
Biophys. J. 90, 1147-1155
   Abstract »    Full Text »    PDF »
Mechanism of Signal Amplification in the Olfactory Sensory Cilia.
H. Takeuchi and T. Kurahashi (2005)
J. Neurosci. 25, 11084-11091
   Abstract »    Full Text »    PDF »
Activation of olfactory-type cyclic nucleotide-gated channels is highly cooperative.
V. Nache, E. Schulz, T. Zimmer, J. Kusch, C. Biskup, R. Koopmann, V. Hagen, and K. Benndorf (2005)
J. Physiol. 569, 91-102
   Abstract »    Full Text »    PDF »
Functional consequences of progressive cone dystrophy-associated mutations in the human cone photoreceptor cyclic nucleotide-gated channel CNGA3 subunit.
C. Liu and M. D. Varnum (2005)
Am J Physiol Cell Physiol 289, C187-C198
   Abstract »    Full Text »    PDF »
Dynamics of Ca2+-Calmodulin-dependent Inhibition of Rod Cyclic Nucleotide-gated Channels Measured by Patch-clamp Fluorometry.
M. C. Trudeau and W. N. Zagotta (2004)
J. Gen. Physiol. 124, 211-223
   Abstract »    Full Text »    PDF »
Whole-cell Recordings and Photolysis of Caged Compounds in Olfactory Sensory Neurons Isolated from the Mouse.
L. Lagostena and A. Menini (2003)
Chem Senses 28, 705-716
   Abstract »    Full Text »    PDF »
Disruption of an Intersubunit Interaction Underlies Ca2+-Calmodulin Modulation of Cyclic Nucleotide-Gated Channels.
J. Zheng, M. D. Varnum, and W. N. Zagotta (2003)
J. Neurosci. 23, 8167-8175
   Abstract »    Full Text »    PDF »
The Ca-activated Cl Channel and its Control in Rat Olfactory Receptor Neurons.
J. Reisert, P. J. Bauer, K.-W. Yau, and S. Frings (2003)
J. Gen. Physiol. 122, 349-364
   Abstract »    Full Text »    PDF »
Functionally Important Calmodulin-binding Sites in Both NH2- and COOH-terminal Regions of the Cone Photoreceptor Cyclic Nucleotide-gated Channel CNGB3 Subunit.
C. Peng, E. D. Rich, C. A. Thor, and M. D. Varnum (2003)
J. Biol. Chem. 278, 24617-24623
   Abstract »    Full Text »    PDF »
Calcium/Calmodulin Modulation of Olfactory and Rod Cyclic Nucleotide-gated Ion Channels.
M. C. Trudeau and W. N. Zagotta (2003)
J. Biol. Chem. 278, 18705-18708
   Abstract »    Full Text »    PDF »
Importance of the CNGA4 channel gene for odor discrimination and adaptation in behaving mice.
K. R. Kelliher, J. Ziesmann, S. D. Munger, R. R. Reed, and F. Zufall (2003)
PNAS 100, 4299-4304
   Abstract »    Full Text »    PDF »
Olfactory Signal Transduction in the Mouse Septal Organ.
M. Ma, X. Grosmaitre, C. L. Iwema, H. Baker, C. A. Greer, and G. M. Shepherd (2003)
J. Neurosci. 23, 317-324
   Abstract »    Full Text »    PDF »
NO upregulation of a cyclic nucleotide-gated channel contributes to calcium elevation in endothelial cells.
J. Zhang, S.-L. Xia, E. R. Block, and J. M. Patel (2002)
Am J Physiol Cell Physiol 283, C1080-C1089
   Abstract »    Full Text »    PDF »
Cyclic Nucleotide-Gated Ion Channels.
U. B. Kaupp and R. Seifert (2002)
Physiol Rev 82, 769-824
   Abstract »    Full Text »    PDF »
Mechanism of calcium/calmodulin inhibition of rod cyclic nucleotide-gated channels.
M. C. Trudeau and W. N. Zagotta (2002)
PNAS 99, 8424-8429
   Abstract »    Full Text »    PDF »
Central Role of the CNGA4 Channel Subunit in Ca2+-Calmodulin-Dependent Odor Adaptation.
S. D. Munger, A. P. Lane, H. Zhong, T. Leinders-Zufall, K.-W. Yau, F. Zufall, and R. R. Reed (2001)
Science 294, 2172-2175
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)