Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Originally published in Science Express on 1 November 2001
Science 16 November 2001:
Vol. 294. no. 5546, pp. 1519 - 1521
DOI: 10.1126/science.1065201
Prev | Table of Contents | Next

Reports

ARR1, a Transcription Factor for Genes Immediately Responsive to Cytokinins

Hiroe Sakai,1* Takashi Honma,1dagger Takashi Aoyama,1 Shusei Sato,2 Tomohiko Kato,2 Satoshi Tabata,2 Atsuhiro Oka1ddagger

Cytokinins are a class of phytohormones involved in various physiological events of plants. The Arabidopsis sensor histidine kinase CRE1 was recently reported to be a cytokinin receptor. We used a steroid-inducible system to show that the transcription factor-type response regulator ARR1 directs transcriptional activation of the ARR6 gene, which responds to cytokinins without de novo protein synthesis. This fact, together with characteristics of ARR1-overexpressing plants and arr1 mutant plants, indicates that the phosphorelay to ARR1, probably from CRE1, constitutes an intracellular signal transduction occurring immediately after cytokinin perception.

1 Laboratory of Molecular Biology, Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
2 Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan
*   These authors contributed equally to this work.

dagger    Deceased 14 July 2001.

ddagger    To whom correspondence should be addressed. E-mail: oka{at}scl.kyoto-u.ac.jp

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Transcriptional Repressor ARR1-SRDX Suppresses Pleiotropic Cytokinin Activities in Arabidopsis.
A. Heyl, E. Ramireddy, W. G. Brenner, M. Riefler, J. Allemeersch, and T. Schmulling (2008)
Plant Physiology 147, 1380-1395
   Abstract »    Full Text »    PDF »
GeBP and GeBP-Like Proteins Are Noncanonical Leucine-Zipper Transcription Factors That Regulate Cytokinin Response in Arabidopsis.
F. Chevalier, D. Perazza, F. Laporte, G. Le Henanff, P. Hornitschek, J.-M. Bonneville, M. Herzog, and G. Vachon (2008)
Plant Physiology 146, 1142-1154
   Abstract »    Full Text »    PDF »
Three Type-B Response Regulators, ARR1, ARR10 and ARR12, Play Essential but Redundant Roles in Cytokinin Signal Transduction Throughout the Life Cycle of Arabidopsis thaliana.
K. Ishida, T. Yamashino, A. Yokoyama, and T. Mizuno (2008)
Plant Cell Physiol. 49, 47-57
   Abstract »    Full Text »    PDF »
Cytokinin Regulates Type-A Arabidopsis Response Regulator Activity and Protein Stability via Two-Component Phosphorelay.
J. P.C. To, J. Deruere, B. B. Maxwell, V. F. Morris, C. E. Hutchison, F. J. Ferreira, G. E. Schaller, and J. J. Kieber (2007)
PLANT CELL 19, 3901-3914
   Abstract »    Full Text »    PDF »
Involvement of hormones and KNOXI genes in early Arabidopsis seedling development.
P. Soucek, P. Klima, A. Rekova, and B. Brzobohaty (2007)
J. Exp. Bot.
   Abstract »    Full Text »    PDF »
Arabidopsis Cytokinin Signaling Pathway.
B. Muller and J. Sheen (2007)
Sci. STKE 2007, cm5
   Abstract »    Full Text »    PDF »
Cytokinin-binding protein (70 kDa): localization in tissues and cells of etiolated maize seedlings and its putative function.
F. A. Brovko, V. S. Vasil'eva, A. O. Shepelyakovskaya, S. Yu. Selivankina, G. R. Kudoyarova, A. V. Nosov, D. A. Moshkov, A. G. Laman, K. M. Boziev, V. V. Kusnetsov, et al. (2007)
J. Exp. Bot. 58, 2479-2490
   Abstract »    Full Text »    PDF »
Isolation and functional characterization of PgTIP1, a hormone-autotrophic cells-specific tonoplast aquaporin in ginseng.
W. Lin, Y. Peng, G. Li, R. Arora, Z. Tang, W. Su, and W. Cai (2007)
J. Exp. Bot. 58, 947-956
   Abstract »    Full Text »    PDF »
Overexpression of a Type-A Response Regulator Alters Rice Morphology and Cytokinin Metabolism.
N. Hirose, N. Makita, M. Kojima, T. Kamada-Nobusada, and H. Sakakibara (2007)
Plant Cell Physiol. 48, 523-539
   Abstract »    Full Text »    PDF »
ARR1 Directly Activates Cytokinin Response Genes that Encode Proteins with Diverse Regulatory Functions.
M. Taniguchi, N. Sasaki, T. Tsuge, T. Aoyama, and A. Oka (2007)
Plant Cell Physiol. 48, 263-277
   Abstract »    Full Text »    PDF »
Type-B ARR Transcription Factors, ARR10 and ARR12, are Implicated in Cytokinin-Mediated Regulation of Protoxylem Differentiation in Roots of Arabidopsis thaliana.
A. Yokoyama, T. Yamashino, Y.-I. Amano, Y. Tajima, A. Imamura, H. Sakakibara, and T. Mizuno (2007)
Plant Cell Physiol. 48, 84-96
   Abstract »    Full Text »    PDF »
Temporary Expression of the TAF10 Gene and its Requirement for Normal Development of Arabidopsis thaliana.
Y. Tamada, K. Nakamori, H. Nakatani, K. Matsuda, S. Hata, T. Furumoto, and K. Izui (2007)
Plant Cell Physiol. 48, 134-146
   Abstract »    Full Text »    PDF »
Spatial and temporal expression of the response regulators ARR22 and ARR24 in Arabidopsis thaliana.
S. Gattolin, M. Alandete-Saez, K. Elliott, Z. Gonzalez-Carranza, E. Naomab, C. Powell, and J. A. Roberts (2006)
J. Exp. Bot. 57, 4225-4233
   Abstract »    Full Text »    PDF »
The ENHANCER OF SHOOT REGENERATION 2 gene in Arabidopsis Regulates CUP-SHAPED COTYLEDON 1 at the Transcriptional Level and Controls Cotyledon Development.
Y. Ikeda, H. Banno, Q.-W. Niu, S. H. Howell, and N.-H. Chua (2006)
Plant Cell Physiol. 47, 1443-1456
   Abstract »    Full Text »    PDF »
The Arabidopsis Histidine Phosphotransfer Proteins Are Redundant Positive Regulators of Cytokinin Signaling.
C. E. Hutchison, J. Li, C. Argueso, M. Gonzalez, E. Lee, M. W. Lewis, B. B. Maxwell, T. D. Perdue, G. E. Schaller, J. M. Alonso, et al. (2006)
PLANT CELL 18, 3073-3087
   Abstract »    Full Text »    PDF »
A subset of Arabidopsis AP2 transcription factors mediates cytokinin responses in concert with a two-component pathway.
A. M. Rashotte, M. G. Mason, C. E. Hutchison, F. J. Ferreira, G. E. Schaller, and J. J. Kieber (2006)
PNAS 103, 11081-11085
   Abstract »    Full Text »    PDF »
Gene Expression Programs during Shoot, Root, and Callus Development in Arabidopsis Tissue Culture.
P. Che, S. Lall, D. Nettleton, and S. H. Howell (2006)
Plant Physiology 141, 620-637
   Abstract »    Full Text »    PDF »
Signs of change: hormone receptors that regulate plant development..
A. Bishopp, A. P. Mahonen, and Y. Helariutta (2006)
Development 133, 1857-1869
   Abstract »    Full Text »    PDF »
A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture.
M. S. Pischke, E. L. Huttlin, A. D. Hegeman, and M. R. Sussman (2006)
Plant Physiology 140, 1255-1278
   Abstract »    Full Text »    PDF »
Multiple Type-B Response Regulators Mediate Cytokinin Signal Transduction in Arabidopsis.
M. G. Mason, D. E. Mathews, D. A. Argyros, B. B. Maxwell, J. J. Kieber, J. M. Alonso, J. R. Ecker, and G. E. Schaller (2005)
PLANT CELL 17, 3007-3018
   Abstract »    Full Text »    PDF »
Pseudo-Response Regulators (PRRs) or True Oscillator Components (TOCs).
T. Mizuno and N. Nakamichi (2005)
Plant Cell Physiol. 46, 677-685
   Abstract »    Full Text »    PDF »
Combinatorial Microarray Analysis Revealing Arabidopsis Genes Implicated in Cytokinin Responses through the His->Asp Phosphorelay Circuitry.
T. Kiba, T. Naitou, N. Koizumi, T. Yamashino, H. Sakakibara, and T. Mizuno (2005)
Plant Cell Physiol. 46, 339-355
   Abstract »    Full Text »    PDF »
Arabidopsis Response Regulator, ARR22, Ectopic Expression of Which Results in Phenotypes Similar to the wol Cytokinin-Receptor Mutant.
T. Kiba, K. Aoki, H. Sakakibara, and T. Mizuno (2004)
Plant Cell Physiol. 45, 1063-1077
   Abstract »    Full Text »    PDF »
Quantitative Trait Loci Associated With Adventitious Shoot Formation in Tissue Culture and the Program of Shoot Development in Arabidopsis.
S. Lall, D. Nettleton, R. DeCook, P. Che, and S. H. Howell (2004)
Genetics 167, 1883-1892
   Abstract »    Full Text »    PDF »
Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1.
E. J. H. Ross, J. M. Stone, C. G. Elowsky, R. Arredondo-Peter, R. V. Klucas, and G. Sarath (2004)
J. Exp. Bot. 55, 1721-1731
   Abstract »    Full Text »    PDF »
In planta functions of the Arabidopsis cytokinin receptor family.
M. Higuchi, M. S. Pischke, A. P. Mahonen, K. Miyawaki, Y. Hashimoto, M. Seki, M. Kobayashi, K. Shinozaki, T. Kato, S. Tabata, et al. (2004)
PNAS 101, 8821-8826
   Abstract »    Full Text »    PDF »
Histidine Kinase Homologs That Act as Cytokinin Receptors Possess Overlapping Functions in the Regulation of Shoot and Root Growth in Arabidopsis.
C. Nishimura, Y. Ohashi, S. Sato, T. Kato, S. Tabata, and C. Ueguchi (2004)
PLANT CELL 16, 1365-1377
   Abstract »    Full Text »    PDF »
Type-B Response Regulators Display Overlapping Expression Patterns in Arabidopsis.
M. G. Mason, J. Li, D. E. Mathews, J. J. Kieber, and G. E. Schaller (2004)
Plant Physiology 135, 927-937
   Abstract »    Full Text »    PDF »
Ehd1, a B-type response regulator in rice, confers short-day promotion of flowering and controls FT-like gene expression independently of Hd1.
K. Doi, T. Izawa, T. Fuse, U. Yamanouchi, T. Kubo, Z. Shimatani, M. Yano, and A. Yoshimura (2004)
Genes & Dev. 18, 926-936
   Abstract »    Full Text »    PDF »
Type-A Arabidopsis Response Regulators Are Partially Redundant Negative Regulators of Cytokinin Signaling.
J. P.C. To, G. Haberer, F. J. Ferreira, J. Deruere, M. G. Mason, G. E. Schaller, J. M. Alonso, J. R. Ecker, and J. J. Kieber (2004)
PLANT CELL 16, 658-671
   Abstract »    Full Text »    PDF »
Comparative Studies on the Type-B Response Regulators Revealing their Distinctive Properties in the His-to-Asp Phosphorelay Signal Transduction of Arabidopsis thaliana.
Y. Tajima, A. Imamura, T. Kiba, Y. Amano, T. Yamashino, and T. Mizuno (2004)
Plant Cell Physiol. 45, 28-39
   Abstract »    Full Text »    PDF »
Identification of Promoter Motifs Involved in the Network of Phytochrome A-Regulated Gene Expression by Combined Analysis of Genomic Sequence and Microarray Data.
M. E. Hudson and P. H. Quail (2003)
Plant Physiology 133, 1605-1616
   Abstract »    Full Text »
Overexpression of a Mutant Basic Helix-Loop-Helix Protein HFR1, HFR1-{Delta}N105, Activates a Branch Pathway of Light Signaling in Arabidopsis.
K.-Y. Yang, Y.-M. Kim, S. Lee, P.-S. Song, and M.-S. Soh (2003)
Plant Physiology 133, 1630-1642
   Abstract »    Full Text »
Phosphorelay-Regulated Degradation of the Yeast Ssk1p Response Regulator by the Ubiquitin-Proteasome System.
N. Sato, H. Kawahara, A. Toh-e, and T. Maeda (2003)
Mol. Cell. Biol. 23, 6662-6671
   Abstract »    Full Text »    PDF »
The Type-A Response Regulator, ARR15, Acts as a Negative Regulator in the Cytokinin-Mediated Signal Transduction in Arabidopsis thaliana.
T. Kiba, H. Yamada, S. Sato, T. Kato, S. Tabata, T. Yamashino, and T. Mizuno (2003)
Plant Cell Physiol. 44, 868-874
   Abstract »    Full Text »    PDF »
Expression Profiling of Cytokinin Action in Arabidopsis.
A. M. Rashotte, S. D.B. Carson, J. P.C. To, and J. J. Kieber (2003)
Plant Physiology 132, 1998-2011
   Abstract »    Full Text »    PDF »
Systematic Trans-Genomic Comparison of Protein Kinases between Arabidopsis and Saccharomyces cerevisiae.
D. Wang, J. F. Harper, and M. Gribskov (2003)
Plant Physiology 132, 2152-2165
   Abstract »    Full Text »    PDF »
Hormonal Control of Cell Proliferation Requires PASTICCINO Genes.
Y. Harrar, Y. Bellec, C. Bellini, and J.-D. Faure (2003)
Plant Physiology 132, 1217-1227
   Abstract »    Full Text »    PDF »
In Vivo and In Vitro Characterization of the ARR11 Response Regulator Implicated in the His-to-Asp Phosphorelay Signal Transduction in Arabidopsis thaliana.
A. Imamura, T. Kiba, Y. Tajima, T. Yamashino, and T. Mizuno (2003)
Plant Cell Physiol. 44, 122-131
   Abstract »    Full Text »    PDF »
The Arabidopsis AtIPT8/PGA22 Gene Encodes an Isopentenyl Transferase That Is Involved in De Novo Cytokinin Biosynthesis.
J. Sun, Q.-W. Niu, P. Tarkowski, B. Zheng, D. Tarkowska, G. Sandberg, N.-H. Chua, and J. Zuo (2003)
Plant Physiology 131, 167-176
   Abstract »    Full Text »    PDF »
An Arabidopsis histidine kinase is essential for megagametogenesis.
M. S. Pischke, L. G. Jones, D. Otsuga, D. E. Fernandez, G. N. Drews, and M. R. Sussman (2002)
PNAS 99, 15800-15805
   Abstract »    Full Text »    PDF »
Global and Hormone-Induced Gene Expression Changes during Shoot Development in Arabidopsis.
P. Che, D. J. Gingerich, S. Lall, and S. H. Howell (2002)
PLANT CELL 14, 2771-2785
   Abstract »    Full Text »    PDF »
Characterization of the ARR15 and ARR16 Response Regulators with Special Reference to the Cytokinin Signaling Pathway Mediated by the AHK4 Histidine Kinase in Roots of Arabidopsis thaliana.
T. Kiba, H. Yamada, and T. Mizuno (2002)
Plant Cell Physiol. 43, 1059-1066
   Abstract »    Full Text »    PDF »
Molecular Structure of the GARP Family of Plant Myb-Related DNA Binding Motifs of the Arabidopsis Response Regulators.
K. Hosoda, A. Imamura, E. Katoh, T. Hatta, M. Tachiki, H. Yamada, T. Mizuno, and T. Yamazaki (2002)
PLANT CELL 14, 2015-2029
   Abstract »    Full Text »    PDF »
Two-Component Signal Transduction Pathways in Arabidopsis.
I. Hwang, H.-C. Chen, and J. Sheen (2002)
Plant Physiology 129, 500-515
   Abstract »    Full Text »    PDF »
Phosphorelay and Transcription Control in Cytokinin Signal Transduction.
J. Sheen (2002)
Science 296, 1650-1652
   Abstract »    Full Text »    PDF »
Cytokinin Signaling in Arabidopsis.
C. E. Hutchison and J. J. Kieber (2002)
PLANT CELL 14, S47-59
   Full Text »    PDF »
Cytokinins. New Insights into a Classic Phytohormone.
G. Haberer and J. J. Kieber (2002)
Plant Physiology 128, 354-362
   Full Text »    PDF »
Plant Two-Component Signaling Systems and the Role of Response Regulators.
J. Lohrmann and K. Harter (2002)
Plant Physiology 128, 363-369
   Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)