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Science 9 November 2001:
Vol. 294. no. 5545, pp. 1340 - 1343
DOI: 10.1126/science.1064629
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Reports

Reconstitution of Physiological Microtubule Dynamics Using Purified Components

Kazuhisa Kinoshita,1* Isabelle Arnal,12dagger Arshad Desai,1 David N. Drechsel,1 Anthony A. Hyman1*

Microtubules are dynamically unstable polymers that interconvert stochastically between polymerization and depolymerization. Compared with microtubules assembled from purified tubulin, microtubules in a physiological environment polymerize faster and transit more frequently between polymerization and depolymerization. These dynamic properties are essential for the functions of the microtubule cytoskeleton during diverse cellular processes. Here, we have reconstituted the essential features of physiological microtubule dynamics by mixing three purified components: tubulin; a microtubule-stabilizing protein, XMAP215; and a microtubule-destabilizing kinesin, XKCM1. This represents an essential first step in the reconstitution of complex microtubule dynamics-dependent processes, such as chromosome segregation, from purified components.

1 Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307, Dresden, Germany.
2 European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
*   To whom correspondence should be addressed. E-mail: kinoshita{at}mpi-cbg.de, hyman{at}mpi-cbg.de

dagger    Present address: Equipe ATIPE, UMR 6026, Université de Rennes 1, Campus de Beaulieu, Bt 13, 35042 Rennes Cedex, France.

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