Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Nanomedicine Summit 2008

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 9 November 2001:
Vol. 294. no. 5545, pp. 1331 - 1334
DOI: 10.1126/science.1065683
Prev | Table of Contents | Next

Reports

Trithorax and dCBP Acting in a Complex to Maintain Expression of a Homeotic Gene

Svetlana Petruk,1* Yurii Sedkov,1* Sheryl Smith,1 Sergei Tillib,1 Vladislav Kraevski,1 Tatsuya Nakamura,1 Eli Canaani,12 Carlo M. Croce,1 Alexander Mazo1dagger

Trithorax (Trx) is a member of the trithorax group (trxG) of epigenetic regulators, which is required to maintain active states of Hox gene expression during development. We have purified from Drosophila embryos a trithorax acetylation complex (TAC1) that contains Trx, dCBP, and Sbf1. Like CBP, TAC1 acetylates core histones in nucleosomes, suggesting that this activity may be important for epigenetic maintenance of gene activity. dCBP and Sbf1 associate with specific sites on salivary gland polytene chromosomes, colocalizing with many Trx binding sites. One of these is the site of the Hox gene Ultrabithorax (Ubx). Mutations in either trx or the gene encoding dCBP reduce expression of the endogenous Ubx gene as well as of transgenes driven by the bxd regulatory region of Ubx. Thus Trx, dCBP, and Sbf1 are closely linked, physically and functionally, in the maintenance of Hox gene expression.

1 Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.
2 Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: A_Mazo{at}lac.jci.tju.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Association of trxG and PcG proteins with the bxd maintenance element depends on transcriptional activity.
S. Petruk, S. T. Smith, Y. Sedkov, and A. Mazo (2008)
Development 135, 2383-2390
   Abstract »    Full Text »    PDF »
The multidomain protein Brpf1 binds histones and is required for Hox gene expression and segmental identity.
K. Laue, S. Daujat, J. G. Crump, N. Plaster, H. H. Roehl, Tubingen 2000 Screen Consortium, C. B. Kimmel, R. Schneider, and M. Hammerschmidt (2008)
Development 135, 1935-1946
   Abstract »    Full Text »    PDF »
The Highly Similar Arabidopsis Homologs of Trithorax ATX1 and ATX2 Encode Proteins with Divergent Biochemical Functions.
A. Saleh, R. Alvarez-Venegas, M. Yilmaz, O. Le, G. Hou, M. Sadder, A. Al-Abdallat, Y. Xia, G. Lu, I. Ladunga, et al. (2008)
PLANT CELL 20, 568-579
   Abstract »    Full Text »    PDF »
Comparing active and repressed expression states of genes controlled by the Polycomb/Trithorax group proteins.
C. Beisel, A. Buness, I. M. Roustan-Espinosa, B. Koch, S. Schmitt, S. A. Haas, M. Hild, T. Katsuyama, and R. Paro (2007)
PNAS 104, 16615-16620
   Abstract »    Full Text »    PDF »
The Arabidopsis homologs of trithorax (ATX1) and enhancer of zeste (CLF) establish 'bivalent chromatin marks' at the silent AGAMOUS locus.
A. Saleh, A. Al-Abdallat, I. Ndamukong, R. Alvarez-Venegas, and Z. Avramova (2007)
Nucleic Acids Res. 35, 6290-6296
   Abstract »    Full Text »    PDF »
Functional Role of CREB-Binding Protein in the Circadian Clock System of Drosophila melanogaster.
C. Lim, J. Lee, C. Choi, J. Kim, E. Doh, and J. Choe (2007)
Mol. Cell. Biol. 27, 4876-4890
   Abstract »    Full Text »    PDF »
Crucial Roles for Chromatin Dynamics in Cellular Memory.
S. Hirose (2007)
J. Biochem. 141, 615-619
   Abstract »    Full Text »    PDF »
Proteolysis of MLL family proteins is essential for Taspase1-orchestrated cell cycle progression.
S. Takeda, D. Y. Chen, T. D. Westergard, J. K. Fisher, J. A. Rubens, S. Sasagawa, J. T. Kan, S. J. Korsmeyer, E. H.-Y. Cheng, and J. J.-D. Hsieh (2006)
Genes & Dev. 20, 2397-2409
   Abstract »    Full Text »    PDF »
Histone trimethylation and the maintenance of transcriptional ONand OFF states by trxG and PcG proteins.
B. Papp and J. Muller (2006)
Genes & Dev. 20, 2041-2054
   Abstract »    Full Text »    PDF »
Multiple epigenetic maintenance factors implicated by the loss of Mll2 in mouse development.
S. Glaser, J. Schaft, S. Lubitz, K. Vintersten, F. van der Hoeven, K. R. Tufteland, R. Aasland, K. Anastassiadis, S.-L. Ang, and A. F. Stewart (2006)
Development 133, 1423-1432
   Abstract »    Full Text »    PDF »
A Genetic Screen Identifies Putative Targets and Binding Partners of CREB-Binding Protein in the Developing Drosophila Eye.
J. Anderson, R. Bhandari, and J. P. Kumar (2005)
Genetics 171, 1655-1672
   Abstract »    Full Text »    PDF »
Regulation of cellular plasticity in Drosophila imaginal disc cells by the Polycomb group, trithorax group and lama genes.
A. Klebes, A. Sustar, K. Kechris, H. Li, G. Schubiger, and T. B. Kornberg (2005)
Development 132, 3753-3765
   Abstract »    Full Text »    PDF »
Menin and MLL cooperatively regulate expression of cyclin-dependent kinase inhibitors.
T. A. Milne, C. M. Hughes, R. Lloyd, Z. Yang, O. Rozenblatt-Rosen, Y. Dou, R. W. Schnepp, C. Krankel, V. A. LiVolsi, D. Gibbs, et al. (2005)
PNAS 102, 749-754
   Abstract »    Full Text »    PDF »
CREB Binding Protein Functions During Successive Stages of Eye Development in Drosophila.
J. P. Kumar, T. Jamal, A. Doetsch, F. R. Turner, and J. B. Duffy (2004)
Genetics 168, 877-893
   Abstract »    Full Text »    PDF »
Leukemia Proto-Oncoprotein MLL Forms a SET1-Like Histone Methyltransferase Complex with Menin To Regulate Hox Gene Expression.
A. Yokoyama, Z. Wang, J. Wysocka, M. Sanyal, D. J. Aufiero, I. Kitabayashi, W. Herr, and M. L. Cleary (2004)
Mol. Cell. Biol. 24, 5639-5649
   Abstract »    Full Text »    PDF »
moz regulates Hox expression and pharyngeal segmental identity in zebrafish.
C. T. Miller, L. Maves, and C. B. Kimmel (2004)
Development 131, 2443-2461
   Abstract »    Full Text »    PDF »
General transcriptional silencing by a Polycomb response element in Drosophila.
A. K. Sengupta, A. Kuhrs, and J. Muller (2004)
Development 131, 1959-1965
   Abstract »    Full Text »    PDF »
Myotubularins, a large disease-associated family of cooperating catalytically active and inactive phosphoinositides phosphatases.
J. Laporte, F. Bedez, A. Bolino, and J.-L. Mandel (2003)
Hum. Mol. Genet. 12, R285-292
   Abstract »    Full Text »    PDF »
Inheritance of Polycomb-dependent chromosomal interactions in Drosophila.
F. Bantignies, C. Grimaud, S. Lavrov, M. Gabut, and G. Cavalli (2003)
Genes & Dev. 17, 2406-2420
   Abstract »    Full Text »    PDF »
ASH1, a Drosophila trithorax group protein, is required for methylation of lysine 4 residues on histone H3.
K. N. Byrd and A. Shearn (2003)
PNAS 100, 11535-11540
   Abstract »    Full Text »    PDF »
Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor.
T. Shimojima, M. Okada, T. Nakayama, H. Ueda, K. Okawa, A. Iwamatsu, H. Handa, and S. Hirose (2003)
Genes & Dev. 17, 1605-1616
   Abstract »    Full Text »    PDF »
Multiple levels of transcriptional and post-transcriptional regulation are required to define the domain of Hoxb4 expression.
T. Brend, J. Gilthorpe, D. Summerbell, and P. W. J. Rigby (2003)
Development 130, 2717-2728
   Abstract »    Full Text »    PDF »
Comparative Analysis of SET Domain Proteins in Maize and Arabidopsis Reveals Multiple Duplications Preceding the Divergence of Monocots and Dicots.
N. M. Springer, C. A. Napoli, D. A. Selinger, R. Pandey, K. C. Cone, V. L. Chandler, H. F. Kaeppler, and S. M. Kaeppler (2003)
Plant Physiology 132, 907-925
   Abstract »    Full Text »    PDF »
Activating Signal Cointegrator 2 Belongs to a Novel Steady-State Complex That Contains a Subset of Trithorax Group Proteins.
Y.-H. Goo, Y. C. Sohn, D.-H. Kim, S.-W. Kim, M.-J. Kang, D.-J. Jung, E. Kwak, N. A. Barlev, S. L. Berger, V. T. Chow, et al. (2003)
Mol. Cell. Biol. 23, 140-149
   Abstract »    Full Text »
The Drosophila SNR1 (SNF5/INI1) Subunit Directs Essential Developmental Functions of the Brahma Chromatin Remodeling Complex.
D. R. Marenda, C. B. Zraly, Y. Feng, S. Egan, and A. K. Dingwall (2003)
Mol. Cell. Biol. 23, 289-305
   Abstract »    Full Text »
Native and Recombinant Polycomb Group Complexes Establish a Selective Block to Template Accessibility To Repress Transcription In Vitro.
I. F. G. King, N. J. Francis, and R. E. Kingston (2002)
Mol. Cell. Biol. 22, 7919-7928
   Abstract »    Full Text »    PDF »
A cellular memory module conveys epigenetic inheritance of hedgehog expression during Drosophila wing imaginal disc development.
C. Maurange and R. Paro (2002)
Genes & Dev. 16, 2672-2683
   Abstract »    Full Text »    PDF »
The Core of the Polycomb Repressive Complex Is Compositionally and Functionally Conserved in Flies and Humans.
S. S. Levine, A. Weiss, H. Erdjument-Bromage, Z. Shao, P. Tempst, and R. E. Kingston (2002)
Mol. Cell. Biol. 22, 6070-6078
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)