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Science 2 November 2001:
Vol. 294. no. 5544, pp. 1098 - 1102
DOI: 10.1126/science.1064719
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Reports

Structural Basis for Recognition of the Intron Branch Site RNA by Splicing Factor 1 

Zhihong Liu,1* Ingrid Luyten,1* Matthew J. Bottomley,1 Ana C. Messias,1 Sophie Houngninou-Molango,2 Remco Sprangers,1 Katia Zanier,1 Angela Krämer,2 Michael Sattler1dagger

During spliceosome assembly, splicing factor 1 (SF1) specifically recognizes the intron branch point sequence (BPS) UACUAAC in the pre-mRNA transcripts. We show that the KH-QUA2 region of SF1 defines an enlarged KH (hn RNP K) fold which is necessary and sufficient for BPS binding. The 3' part of the BPS (UAC), including the conserved branch point adenosine (underlined), is specifically recognized in a hydrophobic cleft formed by the Gly-Pro-Arg-Gly motif and the variable loop of the KH domain. The QUA2 region recognizes the 5' nucleotides of the BPS (ACU). The branch point adenosine acting as the nucleophile in the first biochemical step of splicing is deeply buried. BPS RNA recognition suggests how SF1 may facilitate subsequent formation of the prespliceosomal complex A.

1 European Molecular Biology Laboratory (EMBL), Meyerhofstrabeta e 1, D-69117 Heidelberg, Germany.
2 University of Geneva, Department of Cell Biology, CH-1211 Geneva 4, Switzerland.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: sattler{at}EMBL-Heidelberg.de

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