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Science 26 October 2001:
Vol. 294. no. 5543, pp. 867 - 870
DOI: 10.1126/science.1063827
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Recruitment of Mec1 and Ddc1 Checkpoint Proteins to Double-Strand Breaks Through Distinct Mechanisms

Tae Kondo,1* Tatsushi Wakayama,1* Takahiro Naiki,1* Kunihiro Matsumoto,12 Katsunori Sugimoto1dagger

In response to DNA damage, eukaryotic cells activate checkpoint pathways that arrest cell cycle progression and induce the expression of genes required for DNA repair. In budding yeast, the homothallic switching (HO) endonuclease creates a site-specific double-strand break at the mating type (MAT) locus. Continuous HO expression results in the phosphorylation of Rad53, which is dependent on products of the ataxia telangiectasia mutated-related MEC1 gene and other checkpoint genes, including DDC1, RAD9, and RAD24. Chromatin immunoprecipitation experiments revealed that the Ddc1 protein associates with a region near the MAT locus after HO expression. Ddc1 association required Rad24 but not Mec1 or Rad9. Mec1 also associated with a region near the cleavage site after HO expression, but this association is independent of Ddc1, Rad9, and Rad24. Thus, Mec1 and Ddc1 are recruited independently to sites of DNA damage, suggesting the existence of two separate mechanisms involved in recognition of DNA damage.

1 Division of Biological Science, Graduate School of Science, Nagoya University,
2 CREST, Japan Science and Technology Corporation, Chikusa-ku, Nagoya 464-0814, Japan.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: j46036a{at}nucc.cc.nagoya-u.ac.jp

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