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Science 26 October 2001:
Vol. 294. no. 5543, pp. 846 - 849
DOI: 10.1126/science.1062319
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Reports

Requirement of Borate Cross-Linking of Cell Wall Rhamnogalacturonan II for Arabidopsis Growth

Malcolm A. O'Neill,* Stefan Eberhard, Peter Albersheim, Alan G. Darvill

Turgor-driven plant cell growth depends on wall structure. Two allelic L-fucose-deficient Arabidopsis thaliana mutants (mur1-1 and 1-2) are dwarfed and their rosette leaves do not grow normally. mur1 leaf cell walls contain normal amounts of the cell wall pectic polysaccharide rhamnogalacturonan II (RG-II), but only half exists as a borate cross-linked dimer. The altered structure of mur1 RG-II reduces the rate of formation and stability of this cross-link. Exogenous aqueous borate rescues the defect. The reduced cross-linking of RG-II in dwarf mur1 plants indicates that plant growth depends on wall pectic polysaccharide organization.

Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, University of Georgia, 220 Riverbend Road, Athens, GA 30602-4712, USA.
*   To whom correspondence should be addressed. E-mail: mao{at}ccrc.uga.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)