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Science 5 October 2001:
Vol. 294. no. 5540, pp. 115 - 121
DOI: 10.1126/science.294.5540.115
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Research Articles

Replication Dynamics of the Yeast Genome

M. K. Raghuraman,1*dagger Elizabeth A. Winzeler,3* David Collingwood,2* Sonia Hunt,1 Lisa Wodicka,4ddagger Andrew Conway,5 David J. Lockhart,4§ Ronald W. Davis,6 Bonita J. Brewer,1 Walton L. Fangman1

Oligonucleotide microarrays were used to map the detailed topography of chromosome replication in the budding yeast Saccharomyces cerevisiae. The times of replication of thousands of sites across the genome were determined by hybridizing replicated and unreplicated DNAs, isolated at different times in S phase, to the microarrays. Origin activations take place continuously throughout S phase but with most firings near mid-S phase. Rates of replication fork movement vary greatly from region to region in the genome. The two ends of each of the 16 chromosomes are highly correlated in their times of replication. This microarray approach is readily applicable to other organisms, including humans.

1 Department of Genetics,
2 Department of Mathematics, University of Washington, Seattle, WA 98195, USA.
3 Genomics Institute of the Novartis Research Foundation, 3115 Merryfield Row, San Diego, CA 92121, USA.
4 Affymetrix, 3380 Central Expressway, Santa Clara, CA, USA.
5 Silicon Genetics, 935 Washington Street, San Carlos, CA 94070, USA.
6 Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: raghu{at}u.washington.edu

ddagger    Present address: Aventa Biosciences, 4757 Nexus Centre Drive, Suite 200, San Diego, CA 92121, USA.

§   Present address: The Salk Institute for Biological Studies, Laboratory of Genetics, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

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