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Science 28 September 2001:
Vol. 293. no. 5539, pp. 2462 - 2465
DOI: 10.1126/science.1062560
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Reports

Strand-Specific Postreplicative Processing of Mammalian Telomeres

S. M. Bailey,1 M. N. Cornforth,2 A. Kurimasa,3 D. J. Chen,3 E. H. Goodwin1*

Telomeres are specialized nucleoprotein structures that stabilize the ends of linear eukaryotic chromosomes. In mammalian cells, abrogation of telomeric repeat binding factor TRF2 or DNA-dependent protein kinase (DNA-PK) activity causes end-to-end chromosomal fusion, thus establishing an essential role for these proteins in telomere function. Here we show that TRF2-mediated end-capping occurs after telomere replication. The postreplicative requirement for TRF2 and DNA-PKcs, the catalytic subunit of DNA-PK, is confined to only half of the telomeres, namely, those that were produced by leading-strand DNA synthesis. These results demonstrate a crucial difference in postreplicative processing of telomeres that is linked to their mode of replication.

1 Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87544, USA.
2 Department of Radiation Oncology, University of Texas Medical Branch, Galveston, TX 77550, USA.
3 Cell and Molecular Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
*   To whom correspondence should be addressed. E-mail: egoodwin{at}telomere.lanl.gov

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